|
| Status |
Public on Jun 04, 2020 |
| Title |
Genome-wide CRISPR library screening to identify genes involved in the regulation of the kinetic properties of transcriptional bursting |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Cell-to-cell heterogeneity in gene expression can even be observed in the same type of cells, present in a similar environment. Transcriptional bursting is thought to be one of contributing factors to the heterogeneity, but it remains elusive how the kinetic properties of transcriptional bursting (e.g. burst size, burst frequency, and noise induced by transcriptional bursting) are regulated in mammalian cells. To unbiasedly identify genes regulating the kinetic properties of transcriptional bursting, we performed large-scale CRISPR/-Cas9 based screening and functional analysis, and found that Akt/MAPK signaling pathways are involved in the regulation of the kinetic properties of transcriptional bursting.
|
| |
|
| Overall design |
Genome-wide CRISPR library screening was performed in Nanog, Dnmt3l, Trim28 knockin cell lines, in which GFP and iRFP reporter separately inserted into the two alleles. There are two technical replication in FACS-sorted sampels.
|
| |
|
| Contributor(s) |
Ochiai H, Nikaido I |
| Citation(s) |
32596448 |
| |
| Submission date |
Jun 12, 2019 |
| Last update date |
Jul 15, 2020 |
| Contact name |
Itoshi NIKAIDO |
| E-mail(s) |
[email protected]
|
| Organization name |
RIKEN
|
| Department |
Center for Biosystems Dynamics Research
|
| Lab |
Laboratory for Bioinformatics Research
|
| Street address |
2-1 Hirosawa
|
| City |
Wako |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (10)
|
|
| This SubSeries is part of SuperSeries: |
| GSE132593 |
Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells |
|
| Relations |
| BioProject |
PRJNA548636 |
| SRA |
SRP201375 |
Less...
More...