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| Status |
Public on Oct 15, 2019 |
| Title |
Estrogen response in breast cancer cell line MCF-7 is dependent on NR2F2 [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We show that most binding events of NR2F2 occur together with the ERα binding sites.To address the functional relationship between NR2F2 and ERα, we assessed the role of NR2F2 in oestrogen-induced growth in ER positive cell line MCF-7. The MTT experiment showed that inhibition of NR2F2 prevented the oestrogen-induced proliferation of MCF-7 cells.To further explore the effect of NR2F2 on estrogen response, We expanded our knockdown studies by performing RNA-seq analysis for MCF-7 cells transfected with control or NR2F2 shRNAs with or without E2.
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| Overall design |
Study of gene expression in WT and NR2F2 KO in MCF-7 cells before and after estroge treatment
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| |
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| Contributor(s) |
Jiang G, Wang X |
| Citation(s) |
31588232 |
| |
| Submission date |
Jun 10, 2019 |
| Last update date |
Oct 15, 2019 |
| Contact name |
Guojuan Jiang |
| E-mail(s) |
[email protected]
|
| Phone |
18817821244
|
| Organization name |
Shanghai Jiaotong University
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| Department |
School of Medicine
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| Lab |
State Key Laboratory of Medical Genomics
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| Street address |
Ruijin er road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA548082 |
| SRA |
SRP201147 |
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