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| Status |
Public on Oct 15, 2019 |
| Title |
Differential chromatine state and ER binding potentially induced by NR2F2 depletion. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ERα binding activity largely depends on access to binding sites on chromatin, which is facilitated in part by Pioneer Factors (PFs).We show that most binding events of NR2F2 occur together with the ERα binding sites.To explore whether NR2F2 may act as potential pioneer factor of ER, we performed a series of ChIP-seq genome wide in MCF-7. Since NR2F2 associates with chromatin prior to estrogen treatment and its depletion in MCF-7 cells did not affect ERα expression, we hypothesize NR2F2 may inhibit estrogen-dependent growth by modulating ERα recruitment. We performed ChIP-seq genome wide gainst ERα before and after NR2F2 depletion.Covalent modifications are a main chromatin property.To test whether NR2F2 favoured histone modification deposition on chromatin, we profiled ChIP-Seq of H3K4me1, H3K4me3, and H3K27ac following NR2F2 depletion in oestrogen-starved MCF-7 cells to gain comprehensive histone medication landscape.
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| Overall design |
Examination of 3 histone modifications and ER, FOXA1 binding in WT and NR2F2 KO MCF-7 cells.
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| Contributor(s) |
Jiang G, Wang X |
| Citation(s) |
31588232 |
| |
| Submission date |
Jun 10, 2019 |
| Last update date |
Oct 15, 2019 |
| Contact name |
Guojuan Jiang |
| E-mail(s) |
[email protected]
|
| Phone |
18817821244
|
| Organization name |
Shanghai Jiaotong University
|
| Department |
School of Medicine
|
| Lab |
State Key Laboratory of Medical Genomics
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| Street address |
Ruijin er road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA548080 |
| SRA |
SRP200958 |
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