|
| Status |
Public on May 18, 2019 |
| Title |
RNA-seq of manually sorted transgenically labeled sNPFr-Gal4 and TRH-Gal4 drosphila neurons |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq was used to molecularly characterize two drosophila neuron subtypes with demonstrated roles in the modulation of aggression
|
| |
|
| Overall design |
Small pools (15-60 cells) and single cells were manually sorted from protease digested, acutely dissociated (TRH-Gal4) or pinned (sNPFR-Gal4) fly brains, cDNA was synthesized and amplified using SMART-Seq v4 kit (Takara), and RNA-seq libraries were prepared using Nextera XT kit (Illumina)
|
| |
|
| Contributor(s) |
Okaty B, Alekseyenko O |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 17, 2019 |
| Last update date |
May 20, 2019 |
| Contact name |
Benjamin Okaty |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard Medical School
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
| Samples (13)
|
|
| Relations |
| BioProject |
PRJNA543486 |
| SRA |
SRP198766 |
Less...
More...