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| Status |
Public on Apr 10, 2020 |
| Title |
Co-expression Analysis Reveals Dysregulated miRNAs and miRNA-mRNA Interactions in the Development of Contrast-induced Acute Kidney Injury [miRNA] |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose: The pathogenesis of contrast-induced acute kidney injury (CI-AKI) has not yet been clearly understood. miRNAs are important mediators which normally work by post-transcriptional degradation of target mRNAs. Emerging evidence indicated a number of differentially expressed miRNAs in CI-AKI following intravenous contrast medium injection. However, there exist differences in the pathological mechanisms and incidences of CI-AKI between intravenous and intra-arterial contrast administration. We aimed to investigate the critical roles of dysregulated miRNAs and the correlated mRNAs in the kidney injury following intra-arterial contrast exposure.
Methods: Based on a reliable CI-AKI rat model, we sought to investigate the roles of miRNA-mRNA interactions in the kidney injury following intra-arterial contrast exposure using the Illumina Genome Analyzer IIx.
Results: In the study, 36 differentially expressed mature miRNAs were identified ( fold change > 1.5 and p value < 0.05) in the kidney of CI-AKI rats (n = 3) compared with controls (n = 3), consisting of 23 up-regulated and 13 down-regulated ones. Bioinformatics analysis revealed that Wnt, TGF-β, and AMPK signaling pathways were most likely to be modulated by these dysregulated miRNAs. Around 453 dysregulated genes ( fold change > 2.0 and p value < 0.05) were identified. Integrated analysis revealed 2037 putative miRNA-mRNA pairs with negative correlations. Of which, 6 differential miRNAs and 13 genes were selected for further quantitative real-time polymerase chain reaction validation (n = 6 for each group), and a well correspondence between the 2 techniques was observed.
Conclusions: In conclusion, our present study contributes to the first evidence of miRNA-mRNA regulations in the development of kidney injury following an intra-arterial contrast injection route. These novel findings provide insights into the underlying mechanisms of CI-AKI.
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| Overall design |
Three-month-old male Sprague-Dawley rats were used in this study. Kidney mRNA and miRNA profiles of CI-AKI rats and controls were generated by deep sequencing, in triplicate, using Illumina Illumina Genome Analyzer IIx.
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| Contributor(s) |
Wang Z, Huang M, Bao W |
| Citation(s) |
31306435 |
| |
| Submission date |
May 07, 2019 |
| Last update date |
Apr 10, 2020 |
| Contact name |
Zhiqing Wang |
| E-mail(s) |
[email protected]
|
| Phone |
+8618905911812
|
| Organization name |
900 Hospital of the Joint Logistics Team
|
| Department |
Department of Cardiology
|
| Street address |
NO. 156, North Xierhuan Road
|
| City |
Fuzhou |
| State/province |
Fujian |
| ZIP/Postal code |
350025 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE130797 |
Co-expression Analysis Reveals Dysregulated miRNAs and miRNA-mRNA Interactions in the Development of Contrast-induced Acute Kidney Injury |
|
| Relations |
| BioProject |
PRJNA541454 |
| SRA |
SRP195748 |
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