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| Status |
Public on Mar 22, 2022 |
| Title |
Gene expression data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells [24 hours] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the molecular mechanism of the hypopigmentation observed in Dicer KO mice, Dicer knockdown was realised in vitro in normal C57BL/6 mouse melanocyte Melan-a cells.
Transient transfection of Melan-a cells with siRNA directed against Dicer reduced Dicer protein levels to approximately 40% of that of siScr Melan-a cells 24 hours after transfection. We analyzed the transcriptome of Melan-a cells 24 hours after transfection with siDicer or siScr to understand the molecular mechanisms involved in Dicer-dependent melanocyte migration.
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| Overall design |
We extracted total RNA from mouse melanocytes Melan-a cells 24 hours after transfection of siRNA against Dicer (siDicer) or a siRNA control (siScr) and performed microarray-based transcriptome analysis. Three biological replicates were used for each condition.
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| Contributor(s) |
Bertrand J, Larue L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 24, 2019 |
| Last update date |
Mar 25, 2022 |
| Contact name |
Juliette BERTRAND |
| E-mail(s) |
[email protected]
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| Organization name |
Institut Curie
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| Department |
CNRS UMR3347, INSERM U1021
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| Street address |
Bat 110, Centre universitaire
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| City |
ORSAY Cedex |
| ZIP/Postal code |
91405 |
| Country |
France |
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| Platforms (1) |
| GPL20258 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CDF version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE128757 |
Melan-a cells expressing reduced level of Dicer and control Melan-a cells |
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| Relations |
| BioProject |
PRJNA528798 |
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