|
| Status |
Public on May 08, 2019 |
| Title |
Identifying RNAs bound by Drosophila Zn72D |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Zn72D is an RNA binding protein that alters A-to-I RNA editing and splicing in the fly brain. We performed RNA immunoprecipitation and sequencing (RIP-seq) to identify the RNAs that are bound by Zn72D.
|
| |
|
| Overall design |
We immunprecipitated Zn72D using an anti-GFP antibody in Zn72D-GFP(BDSC50830) fly heads. We extracted RNA from 5% of input lysates and after immunoprecipitation from three replicates total.
|
| |
|
| Contributor(s) |
Sapiro AL, Li JB |
| Citation(s) |
32433963 |
| |
| Submission date |
Feb 15, 2019 |
| Last update date |
Jun 29, 2020 |
| Contact name |
Jin Billy Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Street address |
300 Pasteur Dr, Alway M341
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE126631 |
Zinc finger RNA binding protein Zn72D regulates ADAR-mediated RNA editing in neurons |
|
| Relations |
| BioProject |
PRJNA522777 |
| SRA |
SRP186008 |
Less...
More...