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| Status |
Public on Feb 11, 2019 |
| Title |
The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by B. melitensis m5-90 [mRNA] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We used B. melitensis M5-90 wild type to construct gene-deleted strains of B. melitensis ∆per, RAW264.7 cells infected with B. melitensis M5-90 and B. melitensis M5-90 ∆per for 4h, respectively. miRNA microarray and mRNA array experiments were performed, qRT-PCR validation for miRNAs and mRNAs. We performed a joint analysis of differentially expressed miRNAs and mRNAs, and proved that the target gene of miRNA-146b is Tbc1d14. It further confirmed that miR-146b targeting tbc1d14 regulates brucella-mediated autophagy of RAW264.7 cells. Finally, the molecular mechanism of tbc1d14 influencing brucella-mediated autophagy of RAW264.7 cells was preliminarily revealed by DGE sequencing.
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| Overall design |
In this study，We used B. melitensis M5-90 wild type to construct gene-deleted strains of B. melitensis ∆per, RAW264.7 cells infected with B. melitensis M5-90 and B. melitensis M5-90 ∆per for 4h, respectively. Three samples from each of the two groups were selected for the mRNA microarray.
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| Contributor(s) |
Jiao H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 10, 2019 |
| Last update date |
Feb 13, 2019 |
| Contact name |
Xie qin |
| E-mail(s) |
[email protected]
|
| Phone |
15618926050
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| Organization name |
LC sciences
|
| Street address |
Minchi first road, pujiang town, minhang district, Shanghai
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| City |
shanghai |
| ZIP/Postal code |
200000 |
| Country |
China |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (6)
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| GSM3596912 |
per1 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h |
| GSM3596913 |
per2 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h |
| GSM3596914 |
per3 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h |
| GSM3596915 |
M5_90_1 from RAW264.7 cells infected with B. melitensis M5-90 for 4h |
| GSM3596916 |
M5_90_2 from RAW264.7 cells infected with B. melitensis M5-90 for 4h |
| GSM3596917 |
M5_90_3 from RAW264.7 cells infected with B. melitensis M5-90 for 4h |
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| This SubSeries is part of SuperSeries: |
| GSE126499 |
The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by B. melitensis m5-90 |
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| Relations |
| BioProject |
PRJNA521652 |
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