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| Status |
Public on Feb 07, 2019 |
| Title |
Mismatch-repair signature mutations activate gene enhancers across human colorectal cancer epigenomes |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We show that mismatch-repair (MMR) signature mutations can activate colorectal cancer (CRC)-specific enhancers, through analysis of enhancer DNA associated with the active enhancer histone mark H3K27ac. We demonstrate that MMR mutations activate enhancers using a xenograft metastasis model, where mutations are induced naturally via CRISPR/Cas9 inactivation of MLH1 prior to tumor cell injection.
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| |
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| Overall design |
H3K27ac profiles of LoVo cells, 2 parental WT COLO-205 subclones, 2 MLH1-/- COLO-205 subclones, and 7 xenograft tumors derived from the COLO-205 subclones.
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| |
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| Contributor(s) |
Saiakhova AR, Hung S, Scacheri PC |
| Citation(s) |
30759065 |
| |
| Submission date |
Feb 06, 2019 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Peter Scacheri |
| E-mail(s) |
[email protected]
|
| Phone |
216-368-3458
|
| Organization name |
Case Western Reserve University
|
| Street address |
10900 Euclid Ave; BRB 647
|
| City |
Cleveland Heights |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
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| Platforms (2) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA521260 |
| SRA |
SRP184282 |
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