|
| Status |
Public on May 22, 2020 |
| Title |
iPS derived microglia like cells cultured with human neurons and astrocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this article, Jose Polo and colleagues molecularly compare existing microglia differentiation methods from stem cells, generate a new dual fluorescent and enzymatic reporter tool to study microglia in vitro and in vivo and show that human astrocyte/neuron co-culture pushes the regulatory landscape of in vitro microglia-like cells to more closely resemble bona fide microglia.
|
| |
|
| Overall design |
RNA-seq experiment examination of iPS-derived microglia like cells cultured in presence (or not) of human astrocytes/neurons, or cholesterol
|
| |
|
| Contributor(s) |
Polo JM, Grubman A, Vandekolk TH, Schröder J, Sun G, Hunt C, Haynes JM, Pouton CW |
| Citation(s) |
32442534 |
| |
| Submission date |
Jan 29, 2019 |
| Last update date |
Jun 10, 2020 |
| Contact name |
Jan Schroeder |
| E-mail(s) |
[email protected]
|
| Phone |
0433419028
|
| Organization name |
Monash University
|
| Department |
Anatomy & Developmental Biology
|
| Lab |
Epigenetics & Reprogramming
|
| Street address |
Building 75 Innovation Walk
|
| City |
Clayton |
| State/province |
VIC |
| ZIP/Postal code |
3168 |
| Country |
Australia |
| |
|
| Platforms (1) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA517743 |
| SRA |
SRP182875 |
Less...
More...