|
| Status |
Public on May 13, 2020 |
| Title |
Human Naive Pluripotency Attained by Transient Inhibition of Mtor |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Other
|
| Summary |
The ability to support blastocyst chimera formation is the most critical property of naïve pluripotent stem cells (PSCs). Transient inhibition of mTOR converts human PSCs from primed to naive state. Naïve human PSCs cultured in 2iLI robustly incorporate into mouse embryonic development after injected to mouse blastocysts.
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| |
|
| Overall design |
Quantification of human cells in E17.5 chimeric mouse embryos using next generation squencing of amplicons covering the human and mouse V3 region of 18S rDNA.
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| |
|
| Contributor(s) |
Li H, Hu Z, Jiang H, Ren Y, Yu X, Qiu J, Stablewski AB, Zhang B, Buck MJ, Feng J |
| Citation(s) |
32426495 |
| |
| Submission date |
Jan 29, 2019 |
| Last update date |
Aug 12, 2020 |
| Contact name |
Jian Feng |
| E-mail(s) |
[email protected]
|
| Phone |
716-829-2345
|
| Organization name |
State University of New York at Buffalo
|
| Department |
Department of Physiology and Biophysics
|
| Lab |
Feng Lab
|
| Street address |
955 Main Street, Room 3102
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14203 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
|
| Samples (19)
|
|
| Relations |
| BioProject |
PRJNA517616 |
| SRA |
SRP182788 |
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