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| Status |
Public on Feb 14, 2020 |
| Title |
High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cells differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the mRNA expression of purified tumour-infiltrating M-MDSCs from the NSD or HSD group, we employed Agilent Whole Genome Oligo Microarrays (one-color) as a discovery platform to identify differentially expressed genes (DEGs) between NSD and HSD group. We identified 5391 differentially expressed genes (DEGs) comprising 2586 upregulated and 2805 downregulated genes with a fold change greater than 1.5 between M-MDSCs from the NSD and HSD groups. Subsequently, the results demonstrated a significant shift towards an antitumour pro-inflammatory phenotype in HSD-fed mice with a dominant increase in NOS2, TNF, IL-12a, Toll-like receptor 4 (TLR4) and chemokine (C-C motif) ligand 19 (CCL19) expression and a decrease in lymphocyte antigen 6 complex, locus C1 (Ly6C1), C-C-C motif chemokine ligand 12 (CXCL12), indoleamine 2,3-dioxygenase 1 (IDO1) and CCL2 expression. Among these differences, the difference in the expression of IL-12, TNF-α, NOS2, IL-10 and arginase 1 (Arg1) was confirmed by ELISA or qRT-PCR . Meanwhile, the 2,586 DEGs upregulated (fold change >1.5) in M-MDSCs from the HSD group were markedly enriched in processes involving cytokine production, cell chemotaxis, INF-γ production, cell maturation, osmotic stress responses, acute inflammatory responses, etc. In addition, Analysis of the microarray dataset indicated that the expression of 2 key TFs (activating transcription factor 2 [ATF2] and NFAT5) responsible for osmotic stress was enhanced in the M-MDSCs from the HSD group . We further found that NFAT5 expression was significantly upregulated in M-MDSCs from the HSD group but that there was no difference in ATF2 expression between the two groups in both tumour models.
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| Overall design |
HSD induced gene expression in purified tumour-infiltrating M-MDSCs after HSD treated tumour-bearing mice for 16 days
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| Citation(s) |
32265505 |
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| Submission date |
Jan 22, 2019 |
| Last update date |
Apr 20, 2020 |
| Contact name |
Wei He |
| E-mail(s) |
[email protected]
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| Organization name |
Anhui medical university
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| Street address |
Meishan road
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| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230032 |
| Country |
China |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (2) |
| GSM3573844 |
M-MDSCs isolated from tumour tissues from NSD treated tumour-bearing mice |
| GSM3573845 |
M-MDSCs isolated from tumour tissues from HSD treated tumour-bearing mice |
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| Relations |
| BioProject |
PRJNA516358 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125430_RAW.tar |
4.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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