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| Status |
Public on Oct 01, 2019 |
| Title |
Transcriptional dysregulation by a nucleus-localized aminoacyl-tRNA synthetase is associated with Charcot-Marie-Tooth neuropathy |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Charcot-Marie-Tooth disease (CMT) is a length-dependent peripheral neuropathy. The aminoacyl-tRNA synthetases constitute the largest protein family implicated in CMT. Aminoacyl-tRNA synthetases are predominantly cytoplasmic, but are also present in the nucleus. Here we show that a nuclear function of tyrosyl-tRNA synthetase (TyrRS) is implicated in a Drosophila model of CMT. CMT-causing mutations in TyrRS induce unique conformational changes, which confer capacity for aberrant interactions with transcriptional regulators in the nucleus, leading to transcription factor E2F1 hyperactivation. Using neuronal tissues, we reveal a broad transcriptional regulation network associated with wild-type TyrRS expression, which is disturbed when a CMT-mutant is expressed. Pharmacological inhibition of TyrRS nuclear entry with embelin reduces, whereas genetic nuclear exclusion of mutant TyrRS prevents hallmark phenotypes of CMT in the Drosophila model. These data highlight that this translation factor may contribute to transcriptional regulation in neurons, and suggest a therapeutic target for CMT.
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| Overall design |
To assess the full spectrum of downstream effects triggered by mutant TyrRS, we performed expression profiling on whole brains of controls: nSyb-Gal4>+, TyrRS-WT, TyrRS-WT-NLS, TyrRS-E196K-NLS and the affected condition: TyrRS-E196K flies that were aged for 10 days
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| Contributor(s) |
Bervoets S, Wei N, Erfurth M, Yusein-Myashkova S, Ermanoska B, Mateiu L, Asselbergh B, Bloquel D, Kakad P, Penserga T, Thomas FP, Guergueltcheva V, Tournev I, Godenschwege T, Jordanova A, Yang X |
| Citation(s) |
31695036 |
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| Submission date |
Jan 18, 2019 |
| Last update date |
Jan 15, 2020 |
| Contact name |
Ligia Monica Mateiu |
| E-mail(s) |
[email protected]
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| Phone |
+3232651778
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| Organization name |
VIB
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| Department |
Center for Molecular Neurology
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| Street address |
Universiteitsplein 1
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| City |
Antwerp |
| ZIP/Postal code |
2610 |
| Country |
Belgium |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA515840 |
| SRA |
SRP180191 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125311_normalized_counts.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
| GSE125311_read_counts.txt.gz |
563.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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