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| Status |
Public on Dec 26, 2018 |
| Title |
STAT3 secretion defect results in development abnormally |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Total RNA from embryos (12 days) of STAT3mut/mut, STAT3mut/wt and STAT3wt/wt mice which come from the same womb were extracted using TRIzol reagent (Invitrogen) following the manufacturer’s protocols. RNA quantity, quality and integrity were analyzed with NanoDrop ND-1000 and standard denaturing agarose gel electrophoresis. Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol of Agilent Technology. The Whole Mouse Genome Oligo Microarray (Agilent 4*44k) represents all known genes and transcripts in the mouse genome (Kyoto Encyclopedia of Genes and Genomes, KEGG). All the microarray experiments were performed by Kangchen Biotechnology Limited (Shanghai, China).
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| Overall design |
Gene expression in embryos fromSTAT3-K177K180R mutant mice (wild type, heterozygous, and homozygous) were used for gene expression. Two independent experiments were performed at each group.
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| Contributor(s) |
Xu L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 25, 2018 |
| Last update date |
Dec 27, 2018 |
| Contact name |
Li Xu |
| E-mail(s) |
[email protected]
|
| Phone |
13003691133
|
| Organization name |
ZJTCMU
|
| Street address |
BinWen Street 358#
|
| City |
HangZhou City |
| State/province |
ZheJiang Province |
| ZIP/Postal code |
310053 |
| Country |
China |
| |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (6)
|
| GSM3530262 |
Embryo from homozygous mutant (STAT3-K177K180R) mice 1 |
| GSM3530263 |
Embryo from homozygous mutant (STAT3-K177K180R) mice 2 |
| GSM3530264 |
Embryo from heterozygous mutant (STAT3-K177K180R) mice 1 |
| GSM3530265 |
Embryo from heterozygous mutant (STAT3-K177K180R) mice 2 |
| GSM3530266 |
Embryo from wild type mutant (STAT3-K177K180R) mice 1 |
| GSM3530267 |
Embryo from wild type mutant (STAT3-K177K180R) mice 2 |
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| Relations |
| BioProject |
PRJNA511761 |
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