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| Status |
Public on Aug 12, 2008 |
| Title |
Transcript abundance data from seedlings of wild-type Ws and ged1 (greening after extended darkness 1) mutant |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
An Arabidopsis mutant showing an altered ability to green on illumination after extended periods of darkness has been isolated in a screen for genomes uncoupled (gun) mutants. Following illumination for 24 h, 10-day-old dark-grown mutant seedlings accumulated 5 times more chlorophyll than wild-type seedlings and this was correlated with differences in plastid morphology observed by transmission electron microscopy. The mutant has been named greening after extended darkness 1 (ged1). We used microarrays to detail the global profiles of transcript abundances in the mutant in comparison to the wild type. Microarray analysis showed much lower amounts of transcripts of genes encoding seed storage proteins, oleosins and late embryogenesis abundant (LEA) proteins in 7-day-old seedlings of ged1 compared to wild type. RNA-gel-blot analyses confirmed very low levels of transcripts of seed protein genes in ged1 seedlings grown for 2-10 days in the dark, and showed higher amounts of transcripts of photosynthesis-related genes in illuminated 10-day-old dark-grown ged1 seedlings compared to wild type.
Keywords: Genotype
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| Overall design |
Total RNA samples were extracted from wild-type Ws and ged1 seedlings grown for 5 days in the dark followed by 2 days in the light and hybridised on Affymetrix ATH-121501 arrays. Transcript amounts between wild-type Ws and ged1 seedlings were compared globally.
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| Contributor(s) |
Choy M, Gray JC |
| Citation(s) |
18931353 |
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| Submission date |
Aug 11, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Mun-Kit Choy |
| Organization name |
University of Cambridge
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| Department |
Division of Cardiovascular Medicine
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| Street address |
Box 110, Level 6, ACCI Building, Addenbrooke’s Hospital, Hills Road
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| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 2QQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA113163 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12401_RAW.tar |
12.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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