|
| Status |
Public on Dec 01, 2020 |
| Title |
ChIP of SMC2 at defined cell cycle stages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Investigating binding of the SMC2 protein genome-wide in synchronised HCT116 cells at early S, late S and G2/M
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| |
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| Overall design |
HCT116 cells were synchronised at the G1/S boundary and released either in normal media or in media supplemented with 0.4microM aphidicolin to induce replication stress. Cells were harvested at 4, 8 and 10h post release, corresponding to early S, late S and G2/M. Cells were fixed and ChIP for SMC2 was performed
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| |
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| Contributor(s) |
Nozawa R, Boteva L, Gilbert N |
| Citation missing |
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| |
| Submission date |
Dec 17, 2018 |
| Last update date |
Dec 04, 2020 |
| Contact name |
Lora Boteva |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0) 131 651 8551
|
| Organization name |
University of Edinburgh
|
| Department |
Institute of Genetics and Molecular Medicine
|
| Lab |
Gilbert Lab
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| State/province |
City of Edinburgh, Midlothian |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA510321 |
| SRA |
SRP173585 |
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