|
| Status |
Public on Nov 04, 2008 |
| Title |
Genome-wide analysis of H3K79 dimethylation in MLL-AF4 leukemic bone marrow |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
We created a mouse model where conditional expression of physiologic levels of an Mll-AF4 fusion oncogene induces development of acute lymphoblastic (ALL) or acute myeloid leukemias (AML). ChIP-chip analysis demonstrated increased histone H3 Lysine 79 (H3K79) dimethylation that correlated with Mll-AF4 associated gene expression profiles in murine ALLs, and in human MLL-rearranged leukemias. In addition, human MLL-rearranged ALLs can be distinguished from other ALLs by their genome-wide H3K79 methylation profiles.
Keywords: Cell type comparison
|
| |
|
| Overall design |
DNA was immunoprecipitated with anti-dimethyl-H3K79 antibodies from bone marrow of mice made leukemic by activation of an MLL-AF4 fusion. The ChIP DNA was then hybridized to Affymetrix promoter tiling microarrays.
|
| |
|
| Contributor(s) |
Lemieux ME |
| Citation(s) |
18977325 |
| |
| Submission date |
Aug 06, 2008 |
| Last update date |
Oct 14, 2014 |
| Contact name |
Madeleine E. Lemieux |
| E-mail(s) |
[email protected]
|
| Phone |
617-595-6695
|
| URL |
http://www.bioinfo.ca
|
| Organization name |
Bioinfo
|
| Street address |
275 Main St., Suite 252
|
| City |
Plantagenet |
| State/province |
ON |
| ZIP/Postal code |
K0B 1L0 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL5811 |
[Mm_PromPR] Affymetrix Mouse Promoter 1.0R Array |
|
| Samples (1) |
|
| This SubSeries is part of SuperSeries: |
| GSE12363 |
H3K79 methylation profiles define murine and human MLL-AF4 leukemias |
|
| Relations |
| BioProject |
PRJNA114201 |
