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| Status |
Public on Dec 08, 2018 |
| Title |
Gene expression profiling of mobilized vs. Bone marrow (BM) resident hematopoietic stem and progenitor cells (LSK) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Expression prolile of CWHM-823+tGro-b mobilized (3 mg/kg + 2.5 mg/kg, s.c. injection, timepoint 30 min) HSPC was compared to that of AMD3100 (5 mg/kg, 1 hr after s.c. injection) or G-CSF (100 ug/kg/injection, q12h, 5 days) mobilized as well as BM resident HSPC. Accordingly, the cells were isolated from the BM of untreated or from the peripheral blood (PB) of differentially mobilized mice.
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| Overall design |
Mice were treated with G-CSF (5 days), AMD3100 (1 hr), CWHM-823+tGro-b or PBS. PB or BM was isolated, depleted of red blood cells and enriched for kit (CD117)+ progenitors. LSK cells (HSPC) were then flow sorted from the positive cell fraction.
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| Contributor(s) |
Karpova D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 07, 2018 |
| Last update date |
Dec 10, 2018 |
| Contact name |
Darja Karpova |
| E-mail(s) |
[email protected]
|
| Phone |
+1-314-362-9335
|
| Organization name |
Washington University School of Medicine
|
| Department |
Oncology, BMT
|
| Lab |
John F. DiPersio
|
| Street address |
660 S Euclid Ave
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA508961 |
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