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| Status |
Public on Mar 27, 2019 |
| Title |
Evaluation of Transcriptomic Change in Late-Delayed Radiation following Fractionated Whole Brain Irradiation and High Dose Total Body Irradiation in Non-Human Primates |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Fractionated whole-brain irradiation for the treatment of intracranial neoplasia causes progressive neurodegeneration and neuroinflammation. The long-term consequences of high single dose brain irradiation are unknown. In order to assess the late effects of brain irradiation we have compared transcriptomic gene expression profiles from nonhuman primates (rhesus macaques, Macaca mulatta) receiving single dose total body irradiation to those given fractionated whole brain radiotherapy and control comparators. Gene expression profiles from the dorsolateral prefrontal cortex (Brodmann area 46), hippocampus, and deep white matter (centrum semiovale) were compared. The goals of this study were to identify novel potential molecular effects of radiation-induced brain injury, to evaluate regional differences in the cerebral radiation response, and to assess whether animals receiving high dose total body irradiation demonstrated similar transcriptomic patterns as those observed in animals receiving fractionated whole brain irradiation.
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| Overall design |
3x3 design in which 3 brain regions [dorsolateral prefrontal cortex (brodmann area 46), hippocampus, and deep whiter matter (centrum semiovale)] were evaluated from three treatment groups: thorax-only irradiated control animals (10 Gy, estimated dose to the brain < 0.1 Gy), animals receiving fractionated whole brain irradiation (n=5, 40 Gy, 8x5 Gy fractions; 12 months prior to necropsy) and animals receiving high, single dose, total body irradiation (n=5, 6.75 - 8.05 Gy, 6-9 years prior to necropsy). The effects of brain region and irradiation status on differential gene expression were evaluated.
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| Contributor(s) |
Andrews RN, Dugan GO, Peiffer AM, Hawkins GA, Hanbury DB, Bourland JD, Hampson RE, Deadwyler SA, Hampson RE |
| Citation(s) |
30694733 |
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| Submission date |
Oct 05, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Rachel N Andrews |
| E-mail(s) |
[email protected]
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| Phone |
3367161561
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| Organization name |
Wake Forest University
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| Department |
Pathology, Section on Comparative Medicine
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| Lab |
Cline
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| Street address |
Medical Center Boulevard
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| City |
Winston-Salem |
| State/province |
North Carolina |
| ZIP/Postal code |
27103 |
| Country |
USA |
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| Platforms (1) |
| GPL21120 |
Illumina NextSeq 500 (Macaca mulatta) |
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| Samples (45)
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| GSM3418122 |
Control Animal 2, Prefrontal Cortex |
| GSM3418123 |
Control Animal 2, Hippocampus |
| GSM3418124 |
Control Animal 2, White Matter |
| GSM3418125 |
Control Animal 3, Prefrontal Cortex |
| GSM3418126 |
Control Animal 3, Hippocampus |
| GSM3418127 |
Control Animal 3, White Matter |
| GSM3418128 |
Control Animal 4, Prefrontal Cortex |
| GSM3418129 |
Control Animal 4, Hippocampus |
| GSM3418130 |
Control Animal 4, White Matter |
| GSM3418131 |
Control Animal 5, Prefrontal Cortex |
| GSM3418132 |
Control Animal 5, Hippocampus |
| GSM3418133 |
Control Animal 5, White Matter |
| GSM3418134 |
TBI Animal 1, Prefrontal Cortex |
| GSM3418135 |
TBI Animal 1, Hippocampus |
| GSM3418136 |
TBI Animal 1, White Matter |
| GSM3418137 |
TBI Animal 2, Prefrontal Cortex |
| GSM3418138 |
TBI Animal 2, Hippocampus |
| GSM3418139 |
TBI Animal 2, White Matter |
| GSM3418140 |
TBI Animal 3, Prefrontal Cortex |
| GSM3418141 |
TBI Animal 3, Hippocampus |
| GSM3418142 |
TBI Animal 3, White Matter |
| GSM3418143 |
TBI Animal 4, Prefrontal Cortex |
| GSM3418144 |
TBI Animal 4, Hippocampus |
| GSM3418145 |
TBI Animal 4, White Matter |
| GSM3418146 |
TBI Animal 5, Prefrontal Cortex |
| GSM3418147 |
TBI Animal 5, Hippocampus |
| GSM3418148 |
TBI Animal 5, White Matter |
| GSM3418149 |
fWBI Animal 1, Prefrontal Cortex |
| GSM3418150 |
fWBI Animal 1, Hippocampus |
| GSM3418151 |
fWBI Animal 1, White Matter |
| GSM3418152 |
fWBI Animal 2, Prefrontal Cortex |
| GSM3418153 |
fWBI Animal 2, Hippocampus |
| GSM3418154 |
fWBI Animal 2, White Matter |
| GSM3418155 |
fWBI Animal 3, Prefrontal Cortex |
| GSM3418156 |
fWBI Animal 3, Hippocampus |
| GSM3418157 |
fWBI Animal 3, White Matter |
| GSM3418158 |
fWBI Animal 4, Prefrontal Cortex |
| GSM3418159 |
fWBI Animal 4, Hippocampus |
| GSM3418160 |
fWBI Animal 4, White Matter |
| GSM3418161 |
fWBI Animal 5, Prefrontal Cortex |
| GSM3418162 |
fWBI Animal 5, Hippocampus |
| GSM3418163 |
fWBI Animal 5, White Matter |
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| Relations |
| BioProject |
PRJNA494867 |
| SRA |
SRP163682 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE120901_Counts_All.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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