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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 12, 2008 |
| Title |
Regulation of human endothelial gene expression by miR-126 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Fish, JE, Santoro, MM, Morton, SU, Yu, S, Yeh, RF, Wythe, JD, Ivey, KI, Bruneau, BG, Stainier, DYR, and Srivastava, D. (2008). miR-126 Regulates Angiogenic Signaling and Vascular Integrity. Developmental Cell 15, 272-284.
Precise regulation of the formation, maintenance, and remodeling of the vasculature is required for normal development, tissue response to injury, and tumor progression. How specific microRNAs intersect with and modulate angiogenic signaling cascades is unknown. Here, we identified microRNAs that were enriched in endothelial cells derived from mouse embryonic stem (ES) cells and in developing mouse embryos. We found that miR-126 regulated the response of endothelial cells to VEGF. Additionally, knockdown of miR-126 in zebrafish resulted in loss of vascular integrity and hemorrhage during embryonic development. miR-126 functioned in part by directly repressing negative regulators of the VEGF pathway, including the Sprouty-related protein SPRED1 and phosphoinositol-3 kinase regulatory subunit 2 (PIK3R2/p85-β). Increased expression of Spred1 or inhibition of VEGF signaling in zebrafish resulted in defects similar to miR-126 knockdown. These findings illustrate that a single miRNA can regulate vascular integrity and angiogenesis, providing a new target for modulating vascular formation and function.
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| Overall design |
Human umbilical vein endothelial cells, which express high levels of miR-126, were utilized to identify mRNA targets of miR-126. Cells were electroporated with 15 nmol of an antisense morpholino that blocks the processing of the miR-126 pri-cursor. A non-targeting morpholino (15 nmol) was used as a control. RNA was isolated 72 hr following electroporation and arrays were performed using Affymetrix Human Gene 1.0 ST arrays. Analysis was performed on three biological replicates of control morpholino and miR-126 antisense morpholino transfected cells
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| Contributor(s) |
Fish JE, Yeh R, Srivastava D |
| Citation(s) |
18694566 |
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| Submission date |
Jul 04, 2008 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Jason E. Fish |
| E-mail(s) |
[email protected]
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| Organization name |
J. David Gladstone Institute
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| Department |
Gladstone Institute of Cardiovascular Disease
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| Lab |
Deepak Srivastava
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| Street address |
1650 Owens Street
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM303570 |
HUVEC_control morpholino 72h_replicate1 |
| GSM303571 |
HUVEC_miR-126 antisense morpholino 72h_replicate1 |
| GSM303572 |
HUVEC_control morpholino 72h_replicate2 |
| GSM303573 |
HUVEC_miR-126 antisense morpholino 72h_replicate2 |
| GSM303574 |
HUVEC_control morpholino 72h_replicate3 |
| GSM303575 |
HUVEC_miR-126 antisense morpholino 72h_replicate3 |
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| This SubSeries is part of SuperSeries: |
| GSE12039 |
Regulation of endothelial gene expression by miR-126 in human and zebrafish |
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| Relations |
| BioProject |
PRJNA114301 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12011_RAW.tar |
26.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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