We have performed modular analyses to decipher the global transcriptional response and capture a breadth of distinct immune responses in the lungs and blood of mice infected or challenged with a broad spectrum of infectious pathogens, including parasites (Toxoplasma gondii), bacteria (Burkholderia pseudomallei), viruses (Influenza A virus and Respiratory Syncytial virus (RSV)) and fungi (Candida albicans), or allergens (House dust mite (HDM), systemic and intra-nasal challenge). In a distinct set of infectious diseases, we tested the blood modular transcriptional signatures in mice infected with Plasmodium chabaudi chabaudi (malaria), murine cytomegalovirus (MCMV), Listeria monocytogenes and chronic Burkholderia pseudomallei. We also investigated the transcriptional profiles of sorted CD4 T cells (total CD4+, CD4+ CD44 high and CD4+ CD44 low) from lung and blood samples from mice challenged with HDM allergen. Moreover, we used mice deficient in either Ifnar or Ifngr, or both, to reveal the individual roles of each pathway in controlling disease in mice infected with Toxoplasma gondii.
Overall design
Microarray analysis of lung samples obtained from mice infected or challenged with Toxoplasma gondii, Influenza A virus, Respiratory Syncytial virus (RSV), acute Burkholderia pseudomallei, Candida albicans, and House dust mite (HDM) allergen.
Please note that [1] the sample description field lists the corresponding raw data file. [2] the negative numbers in the non-normalized data matrices suggest background subtraction may have occurred. However, the provided raw data is only available raw data from which all processed data was generated.
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