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| Status |
Public on Mar 01, 2019 |
| Title |
Kidney micro-organoids generated in suspension culture |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Kidney organoids have potential uses in disease modelling, drug screening and regenerative medicine. However, novel cost-effective techniques are needed to enable scale-up production of kidney cell types in vitro. We describe here a modified suspension culture method for the generation of kidney micro-organoids from human pluripotent stem cells. Each micro-organoid contains 6-10 nephrons surrounded by endothelial and stromal populations. Single cell transcriptional profiling confirmed the presence and transcriptional equivalence of all anticipated renal cell types consistent with a previous organoid culture method. Ligand-receptor mapping identified TGFβ signalling between stromal and epithelial cell types as likely to result in fibrotic changes observed with long-term culture. The addition of an ALK4 inhibitor suppressed stromal expansion, maintaining epithelial morphology and improving maturation. This suspension culture micro-organoid methodology resulted in a 3-4-fold increase in final cell yield compared to static culture, thereby representing an economical approach to the production of kidney cells for various biological applications.
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| Overall design |
Single cell RNA-seq from a pool of kidney micro-organoids.
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| Contributor(s) |
Kumar SV, Lonsdale A |
| Citation(s) |
30846463 |
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| Submission date |
Jul 17, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Andrew Lonsdale |
| E-mail(s) |
[email protected]
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| Organization name |
Murdoch Children's Research Institute
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| Street address |
50 Flemington Rd
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| City |
Parkville |
| State/province |
VIC |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA481467 |
| SRA |
SRP154125 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE117211_RAW.tar |
55.9 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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