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| Status |
Public on Aug 01, 2018 |
| Title |
Nuclear actin is required for transcription during Drosophila oogenesis [ChIP-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We demonstrate that actin interacts with essentially all transcribed genes and co-occupies most gene promoters together with RNA polymerase II (Pol II). On highly expressed genes, actin and Pol II can be found also on the gene bodies.
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| Overall design |
chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) of Mrtf-GFP, actin (AC-74 and AC-15) and Pol II phosphorylated at serine 5 (Pol II S5P) in ovaries of wild type (w1118) and Mrtf mutant (mal-dΔ7) flies, where Mrtf expression is abolished in ovaries, as well as in flies ubiquitously expressing GFP-tagged version of Mrtf (tub mal-d3xGFP)
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| Contributor(s) |
Sokolova M |
| Citation(s) |
30384134, 38301887 |
| |
| Submission date |
Jun 27, 2018 |
| Last update date |
Mar 07, 2024 |
| Contact name |
Maria Sokolova |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Helsinki
|
| Department |
Institute of Biotechnology
|
| Lab |
Nuclear Actin Lab
|
| Street address |
Viikinkaari 5
|
| City |
Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
| |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE116365 |
Nuclear actin is required for transcription during Drosophila oogenesis |
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| Relations |
| BioProject |
PRJNA478251 |
| SRA |
SRP151499 |
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