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| Status |
Public on Feb 21, 2018 |
| Title |
Chromatine immunoprecipation of atonal binding sites in Drosophila L3 Brains |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Atonal is a proneural transcription factor expressed in the Drosophila neuroblast cluster known as the inner proliferation center (IPC). To characterize the putative targes of Atonal in the IPC we have used an endogenously GFP-tagged version of atonal to immunoprecipate brain samples with anti-GFP antibodies
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| Overall design |
L3 larvae of the Ato::GFP genotype where dissected to collect developing brains. The fixed sample was homogenized by sonication. A 10% of the sonicated volume was processed as input controls while the rest was Immunoprecipitated with antiGFP antibodies, purified and sequence.
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| Contributor(s) |
Mora N, Fiers M, Hassan B |
| Citation(s) |
29576424 |
| |
| Submission date |
Feb 15, 2018 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Mark Fiers |
| E-mail(s) |
[email protected]
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| Organization name |
VIB
|
| Department |
Center for Brain and Disease
|
| Street address |
Herestraat 49
|
| City |
Leuven |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA434661 |
| SRA |
SRP133106 |
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