|
| Status |
Public on Jan 18, 2018 |
| Title |
Mapping open chromatin in mouse nephron progenitor cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To delineate the epigenomic profile of the Six2+ mouse nephron progenitor cells, we mapped open chromatin using ATAC-Seq in Six2+ cells from E16.5 mouse kidneys.
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| |
|
| Overall design |
Using Six2TGC/+ transgenic mice, we isolated Six2+ cells by FACS enriching for GFP+ cells from dissociated kidney cortex, then use 50,000 cells for each ATAC-Seq reaction following Buenrostro et al, 2013.
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| |
|
| Contributor(s) |
Guo Q, McMahon A |
| Citation(s) |
29377931 |
| |
| Submission date |
Dec 22, 2017 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Andrew P McMahon |
| E-mail(s) |
[email protected]
|
| Phone |
323-442-7847
|
| Organization name |
University of Southern California
|
| Department |
Stem Cell Biol & Regen Med
|
| Lab |
Andrew McMahon
|
| Street address |
1425 San Pablo St, BCC 312
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE90017 |
Transcriptional and cistromic profiling of mouse nephron progenitor cells |
|
| Relations |
| BioProject |
PRJNA427367 |
| SRA |
SRP127486 |
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