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| Status |
Public on Aug 11, 2008 |
| Title |
Global gene expression from SOX7 and SOX17 over-expressing human embryonic stem cells (CA1 and CA2 lines) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This study aimed to understand the transcriptional networks regulating endoderm specification from HESC and therefore explored the phenotype of CA1 and CA2 HESC constitutively over-expressing SOX7 or SOX17. Cell lines were created using an inducible construct whereby clonal populations containing transgene integration are selected by Neomycin resistance without expressing of the gene of interest (NoCre controls). Transgene expression is induced via Cre-mediated recombination and selected for puromycin resistance (SOX O/E). The phenotype of the resulting cells suggests that SOX7 expressing HESC represent stable extraembryonic endoderm progenitors, while SOX17 expressing HESC represent early definitive endoderm progenitors. Both in vitro and in vivo SOX7 expressing HESC are restricted to the extraembryonic endoderm lineage, while SOX17 expressing HESC demonstrate mesendodermal specificity. In vitro, SOX17 expressing HESC efficiently produce mature definitive endoderm derivatives.
The molecular phenotype of the resulting SOX7 and SOX17 expressing HESC was characterized by microarray analysis
Keywords: cell line comparison
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| Overall design |
Total RNA was extracted from confluent monolayer cultures of SOX7 over-expressing HESC, SOX17 over-expressing HESC, and their respective control parental HESC lines (designated NoCre Sox7 and NoCre Sox17).
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| Contributor(s) |
Seguin C, Rossant J |
| Citation(s) |
18682240 |
| |
| Submission date |
Mar 13, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Cheryle Seguin |
| E-mail(s) |
[email protected]
|
| Organization name |
The Hospital for Sick Children
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| Lab |
Rossant
|
| Street address |
101 College St
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA107399 |
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