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| Status |
Public on Oct 03, 2017 |
| Title |
Gene Expression Regulation by Lung Cancer Oncogenes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To characterize gene expression changes induced by oncogenes implicated in human lung adenocarcinoma, we analyzed the whole transcriptome of NIH3T3 cells expressing mutant EGFR (exon 19 deletion) or wild-type EGFR. Expression levels of several genes from this list were validated by quantitative RT-PCR using the same RNA samples.
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| Overall design |
Total RNA expression profiling was performed by MBL Co., Ltd. RNA was isolated and purified from WT-EGFR– and Mut-EGFR– expressing NIH3T3 cells using the AllPrep DNA/RNA Mini kit (Qiagen). Two independent experiments were performed in obtaining T7-cDNA for each sample.
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| Contributor(s) |
Nagatomo I |
| Citation(s) |
30568033 |
| |
| Submission date |
Oct 02, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Izumi Nagatomo |
| E-mail(s) |
[email protected]
|
| Phone |
81-6-6879-3833
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| Organization name |
Osaka University Graduate School of Medicine
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| Department |
Respiratory Medicine and Clinical Immunology
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| Lab |
Respiratory Medicine
|
| Street address |
2-2 Yamada-oka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA412863 |
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