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Series GSE10449 Query DataSets for GSE10449
Status Public on Feb 08, 2008
Title Genetic Analysis of opaque 2 Modifier Loci in Quality Protein Maize
Organism Zea mays
Experiment type Expression profiling by array
Summary Quality Protein Maize (QPM) was created by selecting for genetic modifiers that convert the starchy endosperm of an opaque2 (o2) mutant to a hard, vitreous phenotype. Genetic analysis has shown there are multiple, unlinked o2 modifiers (Opm), but their identity and mode of action are unknown. A microarray hybridization performed with RNA obtained from true breeding o2 progeny with vitreous and opaque kernel phenotypes identified a small group of differentially expressed genes, some of which map at or near the Opm QTLs.
Keywords: Genetic modification
 
Overall design Compared 18 days after pollination endosperm transcript profiles in true breeding modified (vitreous; V) and non-modified (opaque; O) opaque 2 kernels. Used four vitreous biological reps (V1-V4) and four opaque biological reps (O1-O4). Each biological rep is a pool of 25 kernels representing 5 kernels form each of 5 uniform phenotype ears (vitreous or opaque). Four arrays used: Array 1= V1 vs O1, Array 2 = V2 vs O2, Array 3 = V3 vs O3, Array 4 = V4 vs O4
 
Contributor(s) Holding DR, Larkins BA
Citation(s) 18427771
Submission date Feb 08, 2008
Last update date Mar 19, 2012
Contact name David Richard Holding
E-mail(s) [email protected]
Phone 520 621 9154
Organization name University of Arizona
Department Plant Sciences
Lab Brian Larkins
Street address 303 Forbes Building
City Tucson
State/province AZ
ZIP/Postal code 85721
Country USA
 
Platforms (1)
GPL6438 Maize oligonucleotide array 46K version
Samples (4)
GSM264518 V1 cy5, O1 cy3
GSM264519 V2 cy5, O2 cy3
GSM264520 V3 cy3, O2 cy5
Relations
BioProject PRJNA108001

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10449_RAW.tar 25.2 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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