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| Status |
Public on Sep 15, 2020 |
| Title |
mir-284-dependent dynamics of the Drosophila antennal transcriptome during aging |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We isolated mir-284 from a genetic screen for age-dependent axon degeneration in Drosophila melanogaster. mir-284 levels show a steep decline with aging. Therefore we embarked on a transcriptome-wide analysis after mir-284 depletion in aging flies.
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| Overall design |
To shed light on the mir-284-regulated transcriptome, we performed RNA sequencing (RNA-seq) on wild-type (w1118) and mir-284 KO (w1118;;mir-284 KO) antennae at 3 and 25 days of age in three biological replicates (2 biological replicates for mir-284 KO at 25d) on an Illumina NextSeq.
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| Contributor(s) |
Szabo A, Michaels YS, Knapp DJ, Toegel M, Gladman S, Collins CA, Fulga TA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 15, 2017 |
| Last update date |
Sep 15, 2020 |
| Contact name |
Aron Szabo |
| E-mail(s) |
[email protected]
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| Organization name |
Weatherall Institute of Molecular Medicine, University of Oxford
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| Department |
Radcliffe Department of Medicine
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| Lab |
Tudor Fulga lab
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| Street address |
John Radcliffe Hospital, Headley Way
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| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA407466 |
| SRA |
SRP117742 |
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