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| Status |
Public on Jan 31, 2019 |
| Title |
SCD – Stem Cell Differentiation on board the International Space Station [miRNA-seq on miRNAs from exosomes] |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The bone loss observed in astronauts and animal models after spaceflight is attributable to alterations in the bone tissue formation that depends from the continuous remodeling through the activities of bone-resorbing osteoclasts of hematopoietic lineage and bone-forming osteoblasts of mesenchymal origin. This disease is frequent in aged people, but develops much more rapidly in space. Our experiment, selected by ESA (European Space Agency), aimed to determine how human bone marrow mesenchymal stem cells (hBMSCs) react and differentiate in real microgravity, on board the International Space Station, in approx. 2 weeks time.
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| Overall design |
Increased formation of exosomes/macrovescicle was observed in samples on board the ISS. To further investigate cell-cell communication through miRNAs secreted into the medium, exosomes were purified from spent growth media
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| Contributor(s) |
Bradamante S, Rivero D, Barenghi L, Balsamo M, Minardi S, Vitali F, Cavalieri D |
| Citation |
Authors: Bradamante, Silvia; Rivero, Damariz; Barenghi, Livia; Balsamo, Michele; Minardi, Simone Paolo; Vitali, Francesco; Cavalieri, Duccio; Title: SCD -- Stem Cell Differentiation Toward Osteoblast Onboard the International Space Station; Journal: Microgravity Science and Technology; 19 September 2018; Volume 30, Issue 5, pp 713-729; issn: 1875-0494; DOI: 10.1007/s12217-018-9653-2; URL: https://doi.org/10.1007/s12217-018-9653-2
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| Submission date |
Jul 07, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Silvia Bradamante |
| E-mail(s) |
[email protected]
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| Phone |
+390264485030
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| Organization name |
CNR-ISTM, Institute of Molecular Science and Technology
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| Street address |
Via Golgi 19
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| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (9)
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| GSM2696775 |
miRNAs from spent media of ground cells, Osteogenic medium, biological replicate 1 |
| GSM2696776 |
miRNAs from spent media of ground cells, Osteogenic medium, biological replicate 2 |
| GSM2696777 |
miRNAs from spent media of ground cells, Osteogenic medium, biological replicate 3 |
| GSM2696778 |
miRNAs from spent media of flight cells, Standard medium, biological replicate 1 |
| GSM2696779 |
miRNAs from spent media of flight cells, Standard medium, biological replicate 2 |
| GSM2696780 |
miRNAs from spent media of flight cells, Standard medium, biological replicate 3 |
| GSM2696781 |
miRNAs from spent media of flight cells, Osteogenic medium, biological replicate 1 |
| GSM2696782 |
miRNAs from spent media of flight cells, Osteogenic medium, biological replicate 2 |
| GSM2696783 |
miRNAs from spent media of flight cells, Osteogenic medium, biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE100933 |
SCD – Stem Cell Differentiation on board the International Space Station: the problem of human bone loss |
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| Relations |
| BioProject |
PRJNA393458 |
| SRA |
SRP111349 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100932_miRNAs_count_all_samples.txt.gz |
18.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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