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Status
Public on Sep 15, 2010
Title
Micro-CRIBI Mouse Oligo Array (Operon V1.1)
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Mus musculus
Manufacturer
Microcribi - Microarray Service (CRIBI - University of Padova)
Manufacture protocol
This set consists of 13,439 70mer oligonucleotides (Operon V1.1) designed on Mouse Unigene clusters, mainly in the 3'-terminal region. Each oligonucleotide is spotted by our robotic station Biorobotics Microgrid II, in two replicates on a MICROMAX glass slide SuperChip I (Cat No MPS696) provided by PerkinElmer Life Sciences Inc. The deposition is qualitatively assessed by a scan of the spotted slide at 10 micron resolution, using a GSI Lumonics scanner and a protocol that allows the detection of spotted features through the salts derived from the dried solution in which oligonucleotides are dissolved. Microarray slides that meet the quality standards, are washed as recommended by the supplier and the glass slides are stored, ready for use, in the dark under deep vacuum. Probe annotation was updated, respect to Operon annotation, by scanning oligo sequences in three databases: ENSEMBL (version 56), RefSeq (version 38) and UniGene (version 183).
Support
glass
Coating
aminosilane
Web link
http://microcribi.cribi.unipd.it
Contributor(s)
Cagnin S , Pacchioni B , Millino C , Celegato B , De Pitta' C , Tombolan L , Chemello F , Laveder P , Bean C , Martinati F , Martini P , Lanfranchi G
Submission date
Jul 15, 2010
Last update date
Sep 15, 2010
Contact name
Gerolamo Lanfranchi
E-mail(s)
[email protected]
Phone
+39-0498276219
Organization name
University of Padova
Department
CRIBI - Biotechnology Center and Biology Department
Lab
Functional Genomics Lab
Street address
Via U. Bassi, 58/B
City
Padova
ZIP/Postal code
35131
Country
Italy
Samples (38)
GSM566744 , GSM566746 , GSM566747 , GSM566748 , GSM566749 , GSM566750
GSM566751 ,
GSM566753 ,
GSM566760 ,
GSM566761 ,
GSM566763 ,
GSM566764 ,
GSM566777 ,
GSM566778 ,
GSM566779 ,
GSM566781 ,
GSM566782 ,
GSM566783 ,
GSM566784 ,
GSM566786 ,
GSM1273930 ,
GSM1273931 ,
GSM1273932 ,
GSM1273933 ,
GSM1273934 ,
GSM1273935 ,
GSM1273936 ,
GSM1273937 ,
GSM1273938 ,
GSM1273939 ,
GSM1273940 ,
GSM1273941 ,
GSM1273942 ,
GSM1273943 ,
GSM1273944 ,
GSM1273945 ,
GSM1273946 ,
GSM1273947
Series (2)
GSE23244
Microgenomic Analysis in Skeletal Muscle: Expression Signatures of Individual Fast and Slow Myofibers
GSE52676
Differentially expressed genes involved in the regulation of muscle wasting during catabolic conditions
Data table header descriptions
ID
Array_Row
Array row position in CRIBI mouse oligo array V1.1 (Operon V1.1)
Array_Column
Array column position in CRIBI mouse oligo array V1.1 (Operon V1.1)
Spot_Row
Row probe position in sub-array
Spot_Column
Column probe position in sub-array
Description
Oligo probe description
Operon_ID
Oligo Identify code by Operon
GB_ACC
GenBank accession
ORF
EntrezGene Accession
Gene_Symbol
Official Gene SYmbol
CLONE_ID
Riken or Image IDs
SPOT_ID
Alternative spot identifier
Data table
ID
Array_Row
Array_Column
Spot_Row
Spot_Column
Description
Operon_ID
GB_ACC
ORF
Gene_Symbol
CLONE_ID
SPOT_ID
1
1
1
1
1
B037A12_alignement_grids
unknown (37A12)
B037A12_alignement_grids
2
1
1
1
2
B037A12_alignement_grids
unknown (37A12)
B037A12_alignement_grids
3
1
1
1
3
B037A12_alignement_grids
unknown (37A12)
B037A12_alignement_grids
4
1
1
1
4
B037A12_alignement_grids
unknown (37A12)
B037A12_alignement_grids
5
1
1
1
20
A037E12_EMPTY
unknown (37E12)
A037E12_EMPTY
6
1
1
1
21
A037I12_EMPTY
unknown (37I12)
A037I12_EMPTY
7
1
1
1
22
A037M12_EMPTY
unknown (37M12)
A037M12_EMPTY
8
1
1
1
23
A037A24_EMPTY
unknown (37A24)
A037A24_EMPTY
9
1
1
1
24
A037E24_EMPTY
unknown (37E24)
A037E24_EMPTY
10
1
1
1
25
A037I24_EMPTY
unknown (37I24)
A037I24_EMPTY
11
1
1
1
26
A037M24_EMPTY
unknown (37M24)
A037M24_EMPTY
12
1
1
2
1
B037A12_alignement_grids
unknown (37A12)
B037A12_alignement_grids
13
1
1
2
2
001A12_Septin 2
M000102_01
NM_001159717.1
18000
Sept2
14
1
1
2
3
001E12_Immediate early response 2
M000126_01
NM_010499.4
15936
Ier2
15
1
1
2
4
001I12_Selectin, endothelial cell, ligand
M000150_01
NM_009149.2
20340
Glg1
16
1
1
2
5
001M12_Glucose phosphate isomerase 1 complex
M000174_01
XR_030874.1
14751
Gpi1
17
1
1
2
6
001A24_Hepatic lipase
M000108_01
NM_008280.2
15450
Lipc
18
1
1
2
7
001E24_ATPase, Na+/K+ beta 3 polypeptide
M000132_01
NM_007502.4
11933
Atp1b3
19
1
1
2
8
001I24_Eukaryotic translation initiation factor 4, gamma 2
M000156_01
NM_001040131.2
13690
Eif4g2
20
1
1
2
9
001M24_Acidic nuclear phosphoprotein 32
M000180_01
NM_009672.3
11737
Anp32a
Total number of rows: 28560 Table truncated, full table size 2696 Kbytes .
Supplementary data files not provided