| State-specific morphological deformations of the lipid bilayer explain mechanosensitive gating of MscS ion channels. | State-specific morphological deformations of the lipid bilayer explain mechanosensitive gating of MscS ion channels.
Park YC, Reddy B, Bavi N, Perozo E, Faraldo-Gómez JD., Free PMC Article | 03/5/2023 |
| Interaction between mechanosensitive channels embedded in lipid membrane. | Interaction between mechanosensitive channels embedded in lipid membrane.
Zhu L, Zhao W, Yan Y, Liao X, Bourtsalas A, Dan Y, Xiao H, Chen X. | 06/19/2021 |
| inactivation process of wild-type MscS was strongly affected by voltage. The wild-type MscS inactivated at +60 to +80 mV but not at -60 to +40 mV. | Voltage-Dependent Inactivation of MscS Occurs Independently of the Positively Charged Residues in the Transmembrane Domain.
Nomura T, Sokabe M, Yoshimura K., Free PMC Article | 02/4/2017 |
| Mutations in a conserved domain of E. coli MscS to the most conserved superfamily residue leads to kinetic changes. | Mutations in a Conserved Domain of E. coli MscS to the Most Conserved Superfamily Residue Leads to Kinetic Changes.
Malcolm HR, Blount P., Free PMC Article | 05/28/2016 |
| Molecular dynamics and biophysical analyses show that the volume of the pockets and thus the number of lipid acyl chains within them decreases upon channel opening. | The role of lipids in mechanosensation.
Pliotas C, Dahl AC, Rasmussen T, Mahendran KR, Smith TK, Marius P, Gault J, Banda T, Rasmussen A, Miller S, Robinson CV, Bayley H, Sansom MS, Booth IR, Naismith JH., Free PMC Article | 03/26/2016 |
| Data show that trifluoroethanol (TFE) affects mechanosensitive channel of small conductance (MscS) channel gating kinetics in spheroplasts and liposomes. | Unidirectional incorporation of a bacterial mechanosensitive channel into liposomal membranes.
Nomura T, Cox CD, Bavi N, Sokabe M, Martinac B. | 01/16/2016 |
| Data indicate that mechanosensitive channel of small conductance (MscS) protein opens in response to a relief of intrinsic lipid bilayer pressure. | The mechanosensitive channel of small conductance (MscS) functions as a Jack-in-the box.
Malcolm HR, Blount P, Maurer JA., Free PMC Article | 02/14/2015 |
| We report here crystal structures of MscS from E. coli and Helicobacter pylori solubilized in the detergent beta-dodecylmaltoside at resolutions of 4.4 and 4.2 A, respectively. | Open and shut: crystal structures of the dodecylmaltoside solubilized mechanosensitive channel of small conductance from Escherichia coli and Helicobacter pylori at 4.4 Å and 4.1 Å resolutions.
Lai JY, Poon YS, Kaiser JT, Rees DC., Free PMC Article | 08/31/2013 |
| Article reviews the science that led to the isolation and identification of MscS. structure-function relationship of the channel, in particular the structural and biochemical approaches to understanding channel gating. [Review] | Bacterial mechanosensitive channels--MscS: evolution's solution to creating sensitivity in function.
Naismith JH, Booth IR., Free PMC Article | 03/30/2013 |
| Data show that the MscL/MscS threshold ratio dramatically decreased in thinner compared with thicker bilayers. | Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS.
Nomura T, Cranfield CG, Deplazes E, Owen DM, Macmillan A, Battle AR, Constantine M, Sokabe M, Martinac B., Free PMC Article | 08/25/2012 |
| Tension dependencies of both inactivation and recovery rates suggest the spatial scale of the inactivating transition, which guides the modeling of the inactivated state of MscS. | The pathway and spatial scale for MscS inactivation.
Kamaraju K, Belyy V, Rowe I, Anishkin A, Sukharev S., Free PMC Article | 10/29/2011 |
| Both F68S and L111S in MscS substitutions produced severe loss-of-function phenotypes in vivo by increasing the inactivation rate and promoting unusual 'silent' inactivation from the resting state | The tension-transmitting 'clutch' in the mechanosensitive channel MscS.
Belyy V, Anishkin A, Kamaraju K, Liu N, Sukharev S. | 05/3/2010 |
| MscL and MscS are tangible models for studying conformational transitions in membrane proteins driven directly by membrane tension. | State-stabilizing Interactions in Bacterial Mechanosensitive Channel Gating and Adaptation.
Anishkin A, Sukharev S., Free PMC Article | 01/21/2010 |
| determination of structural rearrangements associated with MscS activation in membranes; transition from closed to open state is accompanied by downward tilting of the transmembrane TM1-TM2 hairpin & by expansion, tilt & rotation of the TM3 helices | A structural mechanism for MscS gating in lipid bilayers.
Vásquez V, Sotomayor M, Cordero-Morales J, Schulten K, Perozo E., Free PMC Article | 01/21/2010 |
| study presents a 3.45 angstrom-resolution structure for the MscS channel in an open conformation; structure suggests a molecular mechanism that underlies MscS gating and its decay of conductivity during prolonged activation | The structure of an open form of an E. coli mechanosensitive channel at 3.45 A resolution.
Wang W, Black SS, Edwards MD, Miller S, Morrison EL, Bartlett W, Dong C, Naismith JH, Booth IR., Free PMC Article | 01/21/2010 |
| Transition to this unique mechanosensitive channel state is discussed in terms of existing data. | Pore mutations of the Escherichia coli MscS channel affect desensitization but not ionic preference.
Edwards MD, Bartlett W, Booth IR., Free PMC Article | 01/21/2010 |
| Analysis of the fluorescence properties of purified mutant MscS proteins containing single Trp residues revealed that W16 and W251 are relatively inaccessible, whereas W240 is accessible to quenching agents. | The role of tryptophan residues in the function and stability of the mechanosensitive channel MscS from Escherichia coli.
Rasmussen A, Rasmussen T, Edwards MD, Schauer D, Schumann U, Miller S, Booth IR. | 01/21/2010 |
| The crystal structure of MscS reveals a homoheptamer with three transmembrane segments and a large cytoplasmic domain | An optimized purification and reconstitution method for the MscS channel: strategies for spectroscopical analysis.
Vásquez V, Cortes DM, Furukawa H, Perozo E. | 01/21/2010 |
| the crystal structure of MscS does not represent an open state; MscS gate involves a vapor-lock mechanism where limited changes of geometry or surface polarity can locally switch the regime between water-filled (conducting)& empty (nonconducting) states | Water dynamics and dewetting transitions in the small mechanosensitive channel MscS.
Anishkin A, Sukharev S., Free PMC Article | 01/21/2010 |