| Uncovering a delicate balance between endonuclease RNase III and ribosomal protein S15 in E. coli ribosome assembly. | Uncovering a delicate balance between endonuclease RNase III and ribosomal protein S15 in E. coli ribosome assembly.
Naganathan A, Keltz R, Lyon H, Culver GM., Free PMC Article | 02/5/2022 |
| Based on these findings, we generated a E38A/E65A/Q165A triple mutant of EcRNase III (EcEEQ) that functions as a bacterial Dicer and produces random, heterogeneous 22-nt-siRNA cocktails that are optimal for gene silencing studies | The molecular mechanism of dsRNA processing by a bacterial Dicer.
Jin L, Song H, Tropea JE, Needle D, Waugh DS, Gu S, Ji X., Free PMC Article | 11/30/2019 |
| In vivo RNA cleavage rules and target repertoire of RNase III in Escherichia coli have been reported. | In vivo cleavage rules and target repertoire of RNase III in Escherichia coli.
Altuvia Y, Bar A, Reiss N, Karavani E, Argaman L, Margalit H., Free PMC Article | 06/29/2019 |
| Molecular dynamics simulations provide a mechanism for facilitated product release, in which the Ser33 phosphomonoester forms a salt bridge with the Arg95 guanidinium group, thereby weakening RNase III engagement of product. | Mechanism of Ribonuclease III Catalytic Regulation by Serine Phosphorylation.
Gone S, Alfonso-Prieto M, Paudyal S, Nicholson AW., Free PMC Article | 02/10/2018 |
| PNPase regulates its own expression via a reversible RNase III-independent pathway acting upstream from the RNase III-dependent branch. | RNase III-Independent Autogenous Regulation of Escherichia coli Polynucleotide Phosphorylase via Translational Repression.
Carzaniga T, Dehò G, Briani F., Free PMC Article | 07/25/2015 |
| Authors propose that another major role for the Nus-modified transcription complex in rrn operons is as an RNA chaperone insuring co-ordination of 16S rRNA folding and RNase III processing that results in production of proper 30S ribosome subunits. | Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in Escherichia coli.
Bubunenko M, Court DL, Al Refaii A, Saxena S, Korepanov A, Friedman DI, Gottesman ME, Alix JH., Free PMC Article | 06/22/2013 |
| The proU mRNA is processed by RNase III and this processing is osmoregulated. | RNase III initiates rapid degradation of proU mRNA upon hypo-osmotic stress in Escherichia coli.
Kavalchuk K, Madhusudan S, Schnetz K. | 08/11/2012 |
| findings reveal an RNase III-mediated regulatory pathway that functions to modulate corA expression and, in turn, the influx of metal ions transported by CorA in E. coli. | RNase III controls the degradation of corA mRNA in Escherichia coli.
Lim B, Sim SH, Sim M, Kim K, Jeon CO, Lee Y, Ha NC, Lee K., Free PMC Article | 06/23/2012 |
| Data indicates that RNase III has a more widespread role in cellular RNA processing than previously envisioned. | Analysis of Escherichia coli RNase E and RNase III activity in vivo using tiling microarrays.
Stead MB, Marshburn S, Mohanty BK, Mitra J, Pena Castillo L, Ray D, van Bakel H, Hughes TR, Kushner SR., Free PMC Article | 07/16/2011 |
| Multiple enzymes are involved in the GadY-directed cleavage of the full-length mRNA including the double-strand RNA-specific endoribonuclease RNase III. | RNase III participates in GadY-dependent cleavage of the gadX-gadW mRNA.
Opdyke JA, Fozo EM, Hemm MR, Storz G., Free PMC Article | 03/19/2011 |
| base-pairing of DsrA with the rpoS leader creates an alternative RNase III cleavage site within the rpoS/DsrA duplex | Translational activation by the noncoding RNA DsrA involves alternative RNase III processing in the rpoS 5'-leader.
Resch A, Afonyushkin T, Lombo TB, McDowall KJ, Bläsi U, Kaberdin VR., Free PMC Article | 01/21/2010 |
| N-terminus verified by Edman degradation on complete protein | Expression and characterization of RNase III and Era proteins. Products of the rnc operon of Escherichia coli.
Chen SM, Takiff HE, Barber AM, Dubois GC, Bardwell JC, Court DL. | 11/5/2007 |
| In this report it is shown that endoribonuclease RNase III acts as a post-transcriptional modulator of bolA expression under carbon starvation conditions | Adaptation to carbon starvation: RNase III ensures normal expression levels of bolA1p mRNA and sigma(S).
Freire P, Amaral JD, Santos JM, Arraiano CM. | 01/21/2010 |
| Potential functions of the conserved acidic residues of RNase III are discussed in the context of the crystallographic data and proposed catalytic mechanisms. | Mutational analysis of the nuclease domain of Escherichia coli ribonuclease III. Identification of conserved acidic residues that are important for catalytic function in vitro.
Sun W, Li G, Nicholson AW. | 01/21/2010 |
| This study has characterized the effects of base pair sequence on the cleavage reactivity and binding affinity of a minimal substrate of E.coli RNase III. | Characterization of RNA sequence determinants and antideterminants of processing reactivity for a minimal substrate of Escherichia coli ribonuclease III.
Pertzev AV, Nicholson AW., Free PMC Article | 01/21/2010 |
| A preliminary model is presented for functional roles of two divalent metal ions in the RNase III catalytic mechanism. | Catalytic mechanism of Escherichia coli ribonuclease III: kinetic and inhibitor evidence for the involvement of two magnesium ions in RNA phosphodiester hydrolysis.
Sun W, Pertzev A, Nicholson AW., Free PMC Article | 01/21/2010 |
| Observed role of RNase III in sodB mRNA turnover. | Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB.
Afonyushkin T, Vecerek B, Moll I, Bläsi U, Kaberdin VR., Free PMC Article | 01/21/2010 |