| Here the authors show that Ctf4 tightly dimerizes CMG helicase, with an extensive interface involving Psf2, Cdc45, and Sld5. Interestingly, Ctf4 binds only one Pol alpha-primase. Thus, Ctf4 may have evolved as a trimer to organize two helicases and one Pol alpha-primase into a replication factory. | Ctf4 organizes sister replisomes and Pol α into a replication factory.
Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O'Donnell ME, Li H., Free PMC Article | 03/14/2020 |
| chromatin-derived histone complexes can be bound simultaneously by Mcm2, Pol alpha and the histone chaperone FACT that is also a replisome component. | Histone H2A-H2B binding by Pol α in the eukaryotic replisome contributes to the maintenance of repressive chromatin.
Evrin C, Maman JD, Diamante A, Pellegrini L, Labib K., Free PMC Article | 10/5/2019 |
| ur results support a model whereby parental (H3-H4)2 complexes displaced from nucleosomes by DNA unwinding at replication forks are transferred by the CMG-Ctf4-Polalph acomplex to lagging-strand DNA for nucleosome assembly at the original location. | The Mcm2-Ctf4-Polα Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands.
Gan H, Serra-Cardona A, Hua X, Zhou H, Labib K, Yu C, Zhang Z., Free PMC Article | 04/20/2019 |
| Core Factor's intrinsic mobility correlates well with different conformational states of the Pol I cleft, in addition to the stabilization of either Rrn7 N-terminal domain near Pol I wall or the tandem winged helix domain of A49 at a partially overlapping location. | Structural mechanism of ATP-independent transcription initiation by RNA polymerase I.
Han Y, Yan C, Nguyen THD, Jackobel AJ, Ivanov I, Knutson BA, He Y., Free PMC Article | 03/31/2018 |
| An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-alpha to Mcm2-7. | An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7.
Perez-Arnaiz P, Bruck I, Colbert MK, Kaplan DL., Free PMC Article | 10/21/2017 |
| We conclude that Fob1 and Pol I make independent contributions to establishment of silencing, though Pol I also reinforces Fob1-dependent silencing. | RNA Polymerase I and Fob1 contributions to transcriptional silencing at the yeast rDNA locus.
Buck SW, Maqani N, Matecic M, Hontz RD, Fine RD, Li M, Smith JS., Free PMC Article | 06/10/2017 |
| Collectively, these data demonstrate a novel role for Ccr4-Not in Pol I transcriptional regulation that is required for bridging mTORC1 signaling to ribosomal RNA synthesis. | Ccr4-not regulates RNA polymerase I transcription and couples nutrient signaling to the control of ribosomal RNA biogenesis.
Laribee RN, Hosni-Ahmed A, Workman JJ, Chen H., Free PMC Article | 01/2/2016 |
| Data suggest that DNA-directed DNA polymerase Pols alpha and delta are the primary lagging-strand replicases and Pol epsilon is primarily a leading-strand replicase. | Heterogeneous polymerase fidelity and mismatch repair bias genome variation and composition.
Lujan SA, Clausen AR, Clark AB, MacAlpine HK, MacAlpine DM, Malc EP, Mieczkowski PA, Burkholder AB, Fargo DC, Gordenin DA, Kunkel TA., Free PMC Article | 07/25/2015 |
| Data indicate that a defective DNA polymerase alpha (Polalpha)/DNA primase/chromatin-binding protein Ctf4 complex causes unscheduled strand annealing and fork reversal. | Error-free DNA damage tolerance and sister chromatid proximity during DNA replication rely on the Polα/Primase/Ctf4 Complex.
Fumasoni M, Zwicky K, Vanoli F, Lopes M, Branzei D., Free PMC Article | 06/20/2015 |
| Degradation of specific nuclear proteins occurs in the cytoplasm in Saccharomyces cerevisiae | Degradation of specific nuclear proteins occurs in the cytoplasm in Saccharomyces cerevisiae.
Chen L, Madura K., Free PMC Article | 12/27/2014 |
| Mcm10 plays two important roles as a linker of the MCM helicase and Polalpha at the elongating replication fork--first, to coordinate the activities of these two molecular motors | Alternative mechanisms for coordinating polymerase alpha and MCM helicase.
Lee C, Liachko I, Bouten R, Kelman Z, Tye BK., Free PMC Article | 01/25/2010 |
| Low levels of Pol1p significantly increase recombination in the rDNA array, both between homologues and between sister chromatids. | Low levels of DNA polymerase alpha induce mitotic and meiotic instability in the ribosomal DNA gene cluster of Saccharomyces cerevisiae.
Casper AM, Mieczkowski PA, Gawel M, Petes TD., Free PMC Article | 01/21/2010 |
| Report the 12 A cryo-electron microscopic structure for the complete 14-subunit yeast Pol I, a homology model for the core enzyme, and the crystal structure of the subcomplex A14/43. | Functional architecture of RNA polymerase I.
Kuhn CD, Geiger SR, Baumli S, Gartmann M, Gerber J, Jennebach S, Mielke T, Tschochner H, Beckmann R, Cramer P. | 01/21/2010 |
| In the yeast Saccharomyces cerevisiae, reduced levels of the replicative alpha DNA polymerase result in greatly elevated frequencies of chromosome translocations and chromosome loss. | Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites.
Lemoine FJ, Degtyareva NP, Lobachev K, Petes TD. | 01/21/2010 |
| an N-terminal region of Polalpha (Pol1p) that is evolutionarily conserved among different species interacts with Spt16p-Pob3p and Ctf4p in vivo | A coordinated temporal interplay of nucleosome reorganization factor, sister chromatin cohesion factor, and DNA polymerase alpha facilitates DNA replication.
Zhou Y, Wang TS., Free PMC Article | 01/21/2010 |