| Spt5 orchestrates cryptic transcript suppression and transcriptional directionality. | Spt5 orchestrates cryptic transcript suppression and transcriptional directionality.
An H, Yang H, Lee D., Free PMC Article | 10/31/2024 |
| Emerging Roles of SPT5 in Transcription. | Emerging Roles of SPT5 in Transcription.
Pandey V, Punniyamoorthy S, Pokharel YR. | 10/28/2023 |
| Opposite roles of transcription elongation factors Spt4/5 and Elf1 in RNA polymerase II transcription through B-form versus non-B DNA structures. | Opposite roles of transcription elongation factors Spt4/5 and Elf1 in RNA polymerase II transcription through B-form versus non-B DNA structures.
Xu J, Chong J, Wang D., Free PMC Article | 07/10/2021 |
| Structural basis of nucleosome transcription mediated by Chd1 and FACT. | Structural basis of nucleosome transcription mediated by Chd1 and FACT.
Farnung L, Ochmann M, Engeholm M, Cramer P., Free PMC Article | 06/26/2021 |
| Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription. | Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription.
Rosen GA, Baek I, Friedman LJ, Joo YJ, Buratowski S, Gelles J., Free PMC Article | 02/13/2021 |
| Transcriptional nucleotide misincorporation is not implicated in TC-NER, and moderate eviction of Spt5 and promotion of error-free transcriptional bypass of DNA lesions by Rad26 facilitates TC-NER. | Evidence that Moderate Eviction of Spt5 and Promotion of Error-Free Transcriptional Bypass by Rad26 Facilitates Transcription Coupled Nucleotide Excision Repair.
Selvam K, Ding B, Sharma R, Li S. | 03/28/2020 |
| All together, our results reveal an important role of Rpb5 in the transition from initiation to elongation mediated by the RNA polymerase II, by modulating the Spt5 association, and the backtracking activity of the enzyme. | Rpb5 modulates the RNA polymerase II transition from initiation to elongation by influencing Spt5 association and backtracking.
Martínez-Fernández V, Garrido-Godino AI, Mirón-García MC, Begley V, Fernández-Pévida A, de la Cruz J, Chávez S, Navarro F. | 04/14/2018 |
| Interactions between SPT5 and RNA Polymerase II | Relationships Between RNA Polymerase II Activity and Spt Elongation Factors to Spt- Phenotype and Growth in Saccharomyces cerevisiae.
Cui P, Jin H, Vutukuru MR, Kaplan CD., Free PMC Article | 12/9/2017 |
| Spt4/5 may be important to coordinate the mechanical movement of RNAPII through the nucleosome with co-transcriptional chromatin modifications during transcription. | The elongation factor Spt4/5 regulates RNA polymerase II transcription through the nucleosome.
Crickard JB, Lee J, Lee TH, Reese JC., Free PMC Article | 12/2/2017 |
| Spt5 is crucial for a normal rate of RNA synthesis and distribution of RNAPII over transcription units. | Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation.
Shetty A, Kallgren SP, Demel C, Maier KC, Spatt D, Alver BH, Cramer P, Park PJ, Winston F., Free PMC Article | 09/9/2017 |
| These findings attribute a new function to a domain of Spt4/5 that associates directly with RNAPII, making significant steps towards elucidating the mechanism behind transcriptional control by Spt4/5. | The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.
Blythe AJ, Yazar-Klosinski B, Webster MW, Chen E, Vandevenne M, Bendak K, Mackay JP, Hartzog GA, Vrielink A., Free PMC Article | 07/15/2017 |
| demonstrate that physical and genetic interactions exist between Hot1 and several proteins involved in transcriptional and posttranscriptional processes: for example, transcription co-activator Sub1 and elongation complex Spt4/5 | Hot1 factor recruits co-activator Sub1 and elongation complex Spt4/5 to osmostress genes.
Gomar-Alba M, Del Olmo M. | 07/1/2017 |
| High-resolution crystallographic results demonstrating that distinct structures are formed by the first through third KOW domains of Saccharomyces cerevisiae Spt5. | Structures and Functions of the Multiple KOW Domains of Transcription Elongation Factor Spt5.
Meyer PA, Li S, Zhang M, Yamada K, Takagi Y, Hartzog GA, Fu J., Free PMC Article | 12/5/2015 |
| The 3D structure of SAGA complexes in which the subunit Sgf73 is partially or fully deleted to remove the deubiquitination module revealed the module localized in the vicinity of Gcn5 and Spt7. | Mapping the deubiquitination module within the SAGA complex.
Durand A, Bonnet J, Fournier M, Chavant V, Schultz P. | 10/31/2015 |
| The findings suggest that Spt5 is a key coordinator for holding the RNA polymerase II complex in a closed conformation that is highly competent for transcription elongation but repressive to transcription coupled DNA repair. | Insights into how Spt5 functions in transcription elongation and repressing transcription coupled DNA repair.
Li W, Giles C, Li S., Free PMC Article | 08/23/2014 |
| Data show that the early Pol II elongation factor Spt5 contributes to stable recruitment of the mRNA capping enzymes Cet1, Ceg1, and Abd1 in the initiation-elongation transition of RNA polymerase II. | Cap completion and C-terminal repeat domain kinase recruitment underlie the initiation-elongation transition of RNA polymerase II.
Lidschreiber M, Leike K, Cramer P., Free PMC Article | 11/30/2013 |
| This study demonstrates that the recruitment of the Saccharomyces cerevisiae Paf1 complex to active genes requires a domain of Rtf1 that directly interacts with the Spt4-Spt5 complex. | The recruitment of the Saccharomyces cerevisiae Paf1 complex to active genes requires a domain of Rtf1 that directly interacts with the Spt4-Spt5 complex.
Mayekar MK, Gardner RG, Arndt KM., Free PMC Article | 10/26/2013 |
| Chromatin immunoprecipitation (ChIP) revealed that the Spt5 C-terminal region is required for normal recruitment of pre-mRNA cleavage factor I (CFI) to the 3' ends of Saccharomyces cerevisiae genes. | The spt5 C-terminal region recruits yeast 3' RNA cleavage factor I.
Mayer A, Schreieck A, Lidschreiber M, Leike K, Martin DE, Cramer P., Free PMC Article | 04/28/2012 |
| Spt5p plays both positive and negative roles in transcription by Pol I. | The transcription elongation factor Spt5 influences transcription by RNA polymerase I positively and negatively.
Anderson SJ, Sikes ML, Zhang Y, French SL, Salgia S, Beyer AL, Nomura M, Schneider DA., Free PMC Article | 07/30/2011 |
| Spt5 is recruited to the rDNA early in transcription and propose that it plays an important role in ribosomal RNA synthesis through direct binding to the Pol I complex. | Yeast transcription elongation factor Spt5 associates with RNA polymerase I and RNA polymerase II directly.
Viktorovskaya OV, Appling FD, Schneider DA., Free PMC Article | 07/30/2011 |
| Data provide evidence implicating the yeast DSIF complex (Spt4/5) and RNA polymerase II phosphorylation by Kin28 and Ctk1 in the recruitment of Rpd3S to active genes. | DSIF and RNA polymerase II CTD phosphorylation coordinate the recruitment of Rpd3S to actively transcribed genes.
Drouin S, Laramée L, Jacques PÉ, Forest A, Bergeron M, Robert F., Free PMC Article | 03/12/2011 |
| She2p interacts in vivo with the elongating forms of RNA polymerase II (pol II) via the transcription elongation factor Spt4-Spt5 | Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud.
Shen Z, St-Denis A, Chartrand P., Free PMC Article | 09/20/2010 |
| suppression of spt5 can result from loss of histone H3 lysines 4 or 36 methylation, or reduced recruitment of Chd1 or the Rpd3S complex | Histone H3K4 and K36 methylation, Chd1 and Rpd3S oppose the functions of Saccharomyces cerevisiae Spt4-Spt5 in transcription.
Quan TK, Hartzog GA., Free PMC Article | 04/12/2010 |
| Spt5 CTR suppresses Rad26-independent TCR by serving as a platform for assembly of a multiple protein suppressor complex that is associated with Pol II. | The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair.
Ding B, LeJeune D, Li S., Free PMC Article | 03/15/2010 |
| Data show that Bur1 phosphorylates the Spt5 C-terminal repeat domain (CTD) both in vivo and in isolated elongation complexes in vitro. | Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex.
Liu Y, Warfield L, Zhang C, Luo J, Allen J, Lang WH, Ranish J, Shokat KM, Hahn S., Free PMC Article | 01/21/2010 |