| Cdc34 phosphorylation by PKA and Sch9 provides a direct tether between G1 cell division events and cell growth | Nutrient sensing kinases PKA and Sch9 phosphorylate the catalytic domain of the ubiquitin-conjugating enzyme Cdc34.
Cocklin R, Goebl M., Free PMC Article | 04/2/2015 |
| Cdc34 showed a strong preference for Ub lysine 48 (K48), with lower activity towards lysine 11 (K11) and lysine 63 (K63). | Molecular and structural insight into lysine selection on substrate and ubiquitin lysine 48 by the ubiquitin-conjugating enzyme Cdc34.
Suryadinata R, Holien JK, Yang G, Parker MW, Papaleo E, Šarčević B., Free PMC Article | 01/18/2014 |
| chromosome segregation in cdc6 mutant is caused by the deregulation of spindle dynamics induced via a regulatory network involving the ubiquitin-conjugating enzyme Cdc34, microtubule-associated proteins and the anaphase-promoting complex activator Cdh1. | "Reductional anaphase" in replication-defective cells is caused by ubiquitin-conjugating enzyme Cdc34-mediated deregulation of the spindle.
Khong JH, Zhang T, Gunaratne J, Blackstock W, Surana U. | 02/2/2013 |
| Cdc34 is particularly sensitive to oxidative inactivation, through sequestration of the catalytic cysteine in a disulfide complex with Uba1, by levels of oxidant that do not reduce global ubiquitinylation of proteins. | Oxidative stress responses involve oxidation of a conserved ubiquitin pathway enzyme.
Doris KS, Rumsby EL, Morgan BA., Free PMC Article | 01/26/2013 |
| Through analysis of the phenotype of a mutant missing a highly conserved sequence motif within the catalytic domain of Cdc34, we discovered previously unrecognized levels of regulation | New insight into the role of the Cdc34 ubiquitin-conjugating enzyme in cell cycle regulation via Ace2 and Sic1.
Cocklin R, Heyen J, Larry T, Tyers M, Goebl M., Free PMC Article | 07/16/2011 |
| Data show that proximal amino acids surrounding the lysine residues in Sic1 and Ub are critical for ubiquitination, and that this mechanism is linked to key residues composing the catalytic core of Cdc34 and independent of SCF. | Molecular basis for lysine specificity in the yeast ubiquitin-conjugating enzyme Cdc34.
Sadowski M, Suryadinata R, Lai X, Heierhorst J, Sarcevic B., Free PMC Article | 06/14/2010 |
| The acidic tail of the Cdc34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCdc4. | The acidic tail of the Cdc34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCdc4.
Kleiger G, Hao B, Mohl DA, Deshaies RJ., Free PMC Article | 01/21/2010 |
| The CK2 phosphorylation of catalytic domain of Cdc34 modulates its activity at the G1 to S transition in Saccharomyces cerevisiae. | The CK2 phosphorylation of catalytic domain of Cdc34 modulates its activity at the G1 to S transition in Saccharomyces cerevisiae.
Coccetti P, Tripodi F, Tedeschi G, Nonnis S, Marin O, Fantinato S, Cirulli C, Vanoni M, Alberghina L. | 01/21/2010 |
| In in vitro experiments with single-lysine-containing Cdc34 mutant proteins of Saccharomyces cerevisiae, the SCF-mediated stimulation of autoubiquitination is limited to specific N-terminal lysines modified via an intermolecular mechanism. | SCF E3-mediated autoubiquitination negatively regulates activity of Cdc34 E2 but plays a nonessential role in the catalytic cycle in vitro and in vivo.
Scaglione KM, Bansal PK, Deffenbaugh AE, Kiss A, Moore JM, Korolev S, Cocklin R, Goebl M, Kitagawa K, Skowyra D., Free PMC Article | 01/21/2010 |
| We propose that interactions between Cdc34 approximately Ub and SCF directly activate ubiquitin transfer within a substrate-SCF-Cdc34 approximately Ub ternary complex. | Evaluation of a diffusion-driven mechanism for substrate ubiquitination by the SCF-Cdc34 ubiquitin ligase complex.
Petroski MD, Kleiger G, Deshaies RJ. | 01/21/2010 |
| The acidic loop of CDC34 favorably positions K48 of a substrate-linked ubiquitin to attack SCF bound Cdc34-ubiquitin thioester and thereby enables processive synthesis of K48-linked ubiquitin chains by SCF-Cdc34. | Mechanism of lysine 48-linked ubiquitin-chain synthesis by the cullin-RING ubiquitin-ligase complex SCF-Cdc34.
Petroski MD, Deshaies RJ. | 01/21/2010 |
| studies demonstrate that CK2-mediated phosphorylation of Cdc34 on the acidic tail domain stimulates Cdc34-SCFCdc4 ubiquitination activity and cell cycle progression | Cdc34 C-terminal tail phosphorylation regulates Skp1/cullin/F-box (SCF)-mediated ubiquitination and cell cycle progression.
Sadowski M, Mawson A, Baker R, Sarcevic B., Free PMC Article | 01/21/2010 |