| A ~3.2 A resolution cryo-EM structure of the D. melanogaster spastin hexamer with a polyglutamate peptide bound in its central pore reveals how concurrent nucleotide and substrate binding organizes the conserved spastin pore loops into an ordered network that is allosterically coupled to oligomerization. 2 electropositive loops arranged in a double-helical staircase coordinate the substrate sidechains. | An allosteric network in spastin couples multiple activities required for microtubule severing.
Sandate CR, Szyk A, Zehr EA, Lander GC, Roll-Mecak A., Free PMC Article | 02/22/2020 |
| targeting sequence directs spastin to the surface of lipid droplets. Depletion of the homologous spastin proteins in both flies and worms affects lipid droplet number and triacylglycerol content. | Spastin binds to lipid droplets and affects lipid metabolism.
Papadopoulos C, Orso G, Mancuso G, Herholz M, Gumeni S, Tadepalle N, Jüngst C, Tzschichholz A, Schauss A, Höning S, Trifunovic A, Daga A, Rugarli EI., Free PMC Article | 03/26/2016 |
| analysis of spastin's microtubule-binding properties and comparison with katanin | Spastin's microtubule-binding properties and comparison to katanin.
Eckert T, Le DT, Link S, Friedmann L, Woehlke G., Free PMC Article | 06/29/2013 |
| The spastin is a key regulator of axon regeneration, and initiation of regeneration is extremely sensitive to the spastin copy number. | Normal spastin gene dosage is specifically required for axon regeneration.
Stone MC, Rao K, Gheres KW, Kim S, Tao J, La Rochelle C, Folker CT, Sherwood NT, Rolls MM., Free PMC Article | 05/11/2013 |
| Dendritic growth is regulated by Kruppel-like factor dendritic arbor reduction protein 1 (Dar1) suppressing the expression of microtubule-severing protein spastin. | Differential regulation of dendritic and axonal development by the novel Krüppel-like factor Dar1.
Ye B, Kim JH, Yang L, McLachlan I, Younger S, Jan LY, Jan YN., Free PMC Article | 05/14/2011 |
| spastin is essential for apical domain biogenesis during rhabdomere elongation in Drosophila photoreceptor morphogenesis | Role of spastin in apical domain control along the rhabdomere elongation in Drosophila photoreceptor.
Chen G, League GP, Nam SC., Free PMC Article | 01/15/2011 |
| Exogenous expression of wild-type Drosophila or human spastin rescues behavioral and cellular defects in spastin null flies equivalently. | Functional conservation of human Spastin in a Drosophila model of autosomal dominant-hereditary spastic paraplegia.
Du F, Ozdowski EF, Kotowski IK, Marchuk DA, Sherwood NT., Free PMC Article | 07/26/2010 |
| X ray structure; data support a model in which spastin pulls the C terminus of tubulin through its central pore, generating a mechanical force that destabilizes tubulin-tubulin interactions within the microtubule lattice | Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin.
Roll-Mecak A, Vale RD., Free PMC Article | 01/21/2010 |
| Spastin and Fidgetin are utilized to stimulate microtubule minus-end depolymerization and flux; they concentrate at centrosomes, where they catalyze the turnover of gamma-tubulin. | Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes.
Zhang D, Rogers GC, Buster DW, Sharp DJ., Free PMC Article | 01/21/2010 |
| D-spastin displays ATPase activity and uses energy from ATP hydrolysis to sever and disassemble microtubules; disease mutations abolish or partially interfere with these activities. | The Drosophila homologue of the hereditary spastic paraplegia protein, spastin, severs and disassembles microtubules.
Roll-Mecak A, Vale RD. | 01/21/2010 |
| Here we identify the predicted gene product CG5977 as the Drosophila homolog of the human spastin gene, with much higher sequence similarities than any other related AAA domain protein in the fly. | Identification of the Drosophila melanogaster homolog of the human spastin gene.
Kammermeier L, Spring J, Stierwald M, Burgunder JM, Reichert H. | 01/21/2010 |