| CENP-C functions in centromere assembly, the maintenance of CENP-A asymmetry and epigenetic age in Drosophila germline stem cells. | CENP-C functions in centromere assembly, the maintenance of CENP-A asymmetry and epigenetic age in Drosophila germline stem cells.
Carty BL, Dattoli AA, Dunleavy EM., Free PMC Article | 09/25/2021 |
| Structural basis for centromere maintenance by Drosophila CENP-A chaperone CAL1. | Structural basis for centromere maintenance by Drosophila CENP-A chaperone CAL1.
Medina-Pritchard B, Lazou V, Zou J, Byron O, Abad MA, Rappsilber J, Heun P, Jeyaprakash AA., Free PMC Article | 12/12/2020 |
| Spt6 is a maintenance factor for centromeric CENP-A. | Spt6 is a maintenance factor for centromeric CENP-A.
Bobkov GOM, Huang A, van den Berg SJW, Mitra S, Anselm E, Lazou V, Schunter S, Feederle R, Imhof A, Lusser A, Jansen LET, Heun P., Free PMC Article | 08/29/2020 |
| Targeted De Novo Centromere Formation in Drosophila Reveals Plasticity and Maintenance Potential of CENP-A Chromatin. | Targeted De Novo Centromere Formation in Drosophila Reveals Plasticity and Maintenance Potential of CENP-A Chromatin.
Palladino J, Chavan A, Sposato A, Mason TD, Mellone BG., Free PMC Article | 08/13/2020 |
| The data show that Drosophila CENP-A has no intrinsic specificity for centromeric chromatin and utilizes separate loading mechanisms for its incorporation into centromeric and ectopic sites. This suggests that the specific association and availability of CENP-A interacting factors are the major determinants of CENP-A loading specificity. | Localization of Drosophila CENP-A to non-centromeric sites depends on the NuRD complex.
Demirdizen E, Spiller-Becker M, Förtsch A, Wilhelm A, Corless S, Bade D, Bergner A, Hessling B, Erhardt S., Free PMC Article | 05/16/2020 |
| Modulation of the phosphorylation state of S20 may provide the cells with a means to fine-tune CENP-A levels in order to prevent deleterious loading to extra-centromeric sites. | Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading.
Huang A, Kremser L, Schuler F, Wilflingseder D, Lindner H, Geley S, Lusser A., Free PMC Article | 05/16/2020 |
| CENP-A levels inversely correlate with mitosis duration suggesting crosstalk of CENP-A loading with the regulatory machinery of mitosis. Mitosis length is influenced by the spindle assembly checkpoint (SAC), and authors found that CAL1 interacts with the SAC protein and RZZ complex component Zw10 and thus constitutes the anchor for the recruitment of RZZ. | The checkpoint protein Zw10 connects CAL1-dependent CENP-A centromeric loading and mitosis duration in Drosophila cells.
Pauleau AL, Bergner A, Kajtez J, Erhardt S., Free PMC Article | 02/22/2020 |
| results suggest that, whereas SCF(Ppa) targets the fraction of CENP-A(CID) that is not in complex with Cal1, APC/C(Cdh1) mediates also degradation of the Cal1-CENP-A(CID) complex and, thus, likely contributes to the regulation of centromeric CENP-A(CID) deposition | The E3-ligases SCFPpa and APC/CCdh1 co-operate to regulate CENP-ACID expression across the cell cycle.
Moreno-Moreno O, Torras-Llort M, Azorin F., Free PMC Article | 11/2/2019 |
| Cid duplication (leading to Cid6) within the Drosophila subgenus and show that not only Cid, but also Cenp-C is duplicated in the entire subgenus. | Concurrent Duplication of Drosophila Cid and Cenp-C Genes Resulted in Accelerated Evolution and Male Germline-Biased Expression of the New Copies.
Teixeira JR, Dias GB, Svartman M, Ruiz A, Kuhn GCS. | 09/21/2019 |
| NLP and HMR largely co-localize in domains that are immediately adjacent to, yet distinct from centromere domains defined by the centromeric histone dCENP-A. | Oligomerization of Drosophila Nucleoplasmin-Like Protein is required for its centromere localization.
Anselm E, Thomae AW, Jeyaprakash AA, Heun P., Free PMC Article | 06/22/2019 |
| transcription-mediated chromatin remodeling is required for the transition of dCENP-A to fully incorporated nucleosomes at the centromere. | Centromere transcription allows CENP-A to transit from chromatin association to stable incorporation.
Bobkov GOM, Gilbert N, Heun P., Free PMC Article | 05/18/2019 |
| ATP synthase F1 subunits recruited to centromeres by CENP-A are required for male meiosis. | ATP synthase F(1) subunits recruited to centromeres by CENP-A are required for male meiosis.
Collins CM, Malacrida B, Burke C, Kiely PA, Dunleavy EM., Free PMC Article | 12/22/2018 |
| centromere assembly factors CAL1 and CENP-C are required for meiotic chromosome segregation, CENP-A assembly and maintenance on sperm, as well as fertility | Nucleolar activity and CENP-C regulate CENP-A and CAL1 availability for centromere assembly in meiosis.
Kwenda L, Collins CM, Dattoli AA, Dunleavy EM., Free PMC Article | 09/10/2016 |
| identify two co-evolving regions, CENP-A L1 and the CAL1 N terminus, as critical for lineage-specific CENP-A incorporation | Co-evolving CENP-A and CAL1 Domains Mediate Centromeric CENP-A Deposition across Drosophila Species.
Rosin L, Mellone BG., Free PMC Article | 09/3/2016 |
| A novel role has been described for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster. | A novel role for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster.
Boltengagen M, Huang A, Boltengagen A, Trixl L, Lindner H, Kremser L, Offterdinger M, Lusser A., Free PMC Article | 08/13/2016 |
| CENP-A assembly by its loading factor, CAL1, requires RNAPII-mediated transcription of the underlying DNA | Establishment of Centromeric Chromatin by the CENP-A Assembly Factor CAL1 Requires FACT-Mediated Transcription.
Chen CC, Bowers S, Lipinszki Z, Palladino J, Trusiak S, Bettini E, Rosin L, Przewloka MR, Glover DM, O'Neill RJ, Mellone BG., Free PMC Article | 10/3/2015 |
| The amount of Cid that is loaded during each cell cycle appears to be determined primarily by the preexisting centromeric Cid, with little flexibility for compensation of accidental losses. | Transgenerational propagation and quantitative maintenance of paternal centromeres depends on Cid/Cenp-A presence in Drosophila sperm.
Raychaudhuri N, Dubruille R, Orsi GA, Bagheri HC, Loppin B, Lehner CF., Free PMC Article | 05/25/2013 |
| CID nucleosomes are octameric in vivo and that CID dimerization is essential for correct centromere assembly. | Assembly of Drosophila centromeric nucleosomes requires CID dimerization.
Zhang W, Colmenares SU, Karpen GH., Free PMC Article | 04/7/2012 |
| The F box protein partner of paired (Ppa) mediates CenH3(CID) stability in Drosophila. Ppa depletion results in increased CenH3(CID) levels. Ppa physically interacts with CenH3(CID) through the CATD(CID) that mediates Ppa-dependent CenH3(CID) stability. | The F box protein partner of paired regulates stability of Drosophila centromeric histone H3, CenH3(CID).
Moreno-Moreno O, Medina-Giró S, Torras-Llort M, Azorín F. | 02/11/2012 |
| Drosophila CENP-C is essential for centromere identity. | Drosophila CENP-C is essential for centromere identity.
Orr B, Sunkel CE. | 04/23/2011 |
| Proteasome-mediated degradation restricts localization of Drosophila CENP-A (CID) to centromeres by eliminating mislocalized CID as well as by regulating available CID levels. | Proteolysis restricts localization of CID, the centromere-specific histone H3 variant of Drosophila, to centromeres.
Moreno-Moreno O, Torras-Llort M, Azorín F., Free PMC Article | 01/21/2010 |
| Study shows by quantitative fluorescence measurements in living early embryos that functional fluorescent fusion proteins of the Drosophila CENP-A and CENP-C homologs are rapidly incorporated into centromeres during anaphase. | Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase.
Schuh M, Lehner CF, Heidmann S. | 01/21/2010 |
| Cid allows for contacts betwen DNA and histones and specific targeting | Centromere targeting element within the histone fold domain of Cid.
Vermaak D, Hayden HS, Henikoff S., Free PMC Article | 01/21/2010 |
| Results suggest that CID mislocalization promotes formation of ectopic centromeres and multicentric chromosomes, which causes chromosome missegregation, aneuploidy, and growth defects. | Mislocalization of the Drosophila centromere-specific histone CID promotes formation of functional ectopic kinetochores.
Heun P, Erhardt S, Blower MD, Weiss S, Skora AD, Karpen GH., Free PMC Article | 01/21/2010 |