| Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers. | Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers.
Deng T, Su S, Yuan X, He J, Huang Y, Ma J, Wang J., Free PMC Article | 08/30/2023 |
| Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD. | Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD.
Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li X, Huang Y, Wang HW, Ma J., Free PMC Article | 07/23/2022 |
| Loquacious-PD regulates the terminus-dependent molecular recognition of Dicer-2 toward double-stranded RNA. | Loquacious-PD regulates the terminus-dependent molecular recognition of Dicer-2 toward double-stranded RNA.
Jonely M, Singh RK, Donelick HM, Bass BL, Noriega R., Free PMC Article | 12/11/2021 |
| We also found that a base-mismatch can enable Loqs-PB to tune miRNA length. We speculate that Loqs-PB tunes miRNA length by changing the conformation of base-mismatched nucleotides in the pre-miRNA stem to that of base-matched ones. | Dicer partner protein tunes the length of miRNAs using base-mismatch in the pre-miRNA stem.
Zhu L, Kandasamy SK, Fukunaga R., Free PMC Article | 07/20/2019 |
| studies indicate that the 22 amino acids at the C terminus of Loqs-PD, including an FDF-like motif, directly interact with the Hel2 subdomain of Dicer-2's helicase domain. This interaction is RNA-independent, but we find that modulation of Dicer-2 cleavage also requires dsRNA binding by Loqs-PD. | Loquacious-PD facilitates Drosophila Dicer-2 cleavage through interactions with the helicase domain and dsRNA.
Trettin KD, Sinha NK, Eckert DM, Apple SE, Bass BL., Free PMC Article | 06/9/2018 |
| Loqs-PD removes the inhibitory effect of inorganic phosphate on Dicer-2 processing of blunt-end hairpin RNAs, allowing siRNA production in the presence of inorganic phosphate. | Loquacious-PD removes phosphate inhibition of Dicer-2 processing of hairpin RNAs into siRNAs.
Fukunaga R., Free PMC Article | 04/14/2018 |
| binding preference for the thermodynamically more stable siRNA end by Loqs-PD alone is greatly amplified in complex with Dcr-2 to initiate strand discrimination by asymmetry sensing in the RISC loading complex. | Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference.
Tants JN, Fesser S, Kern T, Stehle R, Geerlof A, Wunderlich C, Juen M, Hartlmüller C, Böttcher R, Kunzelmann S, Lange O, Kreutz C, Förstemann K, Sattler M., Free PMC Article | 12/30/2017 |
| Loqs may have roles in assisting the de novo emergence of miRNA genes by facilitating dicing of suboptimal hairpin substrates. | The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site.
Lim MY, Ng AW, Chou Y, Lim TP, Simcox A, Tucker-Kellogg G, Okamura K., Free PMC Article | 11/18/2017 |
| Partial reconstitution of the RNAi response in human cells has been demonstrated using Drosophila gene products, DCR-2, Loqs-PD, and R2D2. | Partial reconstitution of the RNAi response in human cells using Drosophila gene products.
Kennedy EM, Kornepati AV, Bogerd HP, Cullen BR., Free PMC Article | 09/9/2017 |
| Loqs binds to dmDcr1 as a monomer using the identified dimerization surface, which suggests that Loqs might form dimers under conditions where dmDcr1 is absent or not accessible | Structural and functional insights into the fly microRNA biogenesis factor Loquacious.
Jakob L, Treiber T, Treiber N, Gust A, Kramm K, Hansen K, Stotz M, Wankerl L, Herzog F, Hannus S, Grohmann D, Meister G., Free PMC Article | 07/30/2016 |
| Data show that dmDcr-2's partner protein, Loquacious-PD, alters termini dependence, enabling dmDcr-2 to cleave substrates normally refractory to cleavage. | Drosophila dicer-2 cleavage is mediated by helicase- and dsRNA termini-dependent states that are modulated by Loquacious-PD.
Sinha NK, Trettin KD, Aruscavage PJ, Bass BL., Free PMC Article | 08/1/2015 |
| R2D2 and Loqs-PD have partially overlapping function. | Transposon defense by endo-siRNAs, piRNAs and somatic pilRNAs in Drosophila: contributions of Loqs-PD and R2D2.
Mirkovic-Hösle M, Förstemann K., Free PMC Article | 09/20/2014 |
| Alternative splicing of the loquacious (loqs)mRNA generates three distinct Dicer partner proteins. | Dicer partner proteins tune the length of mature miRNAs in flies and mammals.
Fukunaga R, Han BW, Hung JH, Xu J, Weng Z, Zamore PD., Free PMC Article | 01/12/2013 |
| Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila, with Loqs primarily required for dsRNA processing. | Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila.
Marques JT, Kim K, Wu PH, Alleyne TM, Jafari N, Carthew RW., Free PMC Article | 03/8/2010 |
| Biogenesis of endo-siRNAs depends on a new isoform of the dsRNA-binding domain protein Loquacious, which is distinct from the isoform acting in the miRNA pathway. | Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced, high-copy sequences.
Hartig JV, Esslinger S, Böttcher R, Saito K, Förstemann K., Free PMC Article | 01/21/2010 |
| Drosophila Rax (dRax) is important for embryonic development. | RAX is required for fly neuronal development and mouse embryogenesis.
Bennett RL, Blalock WL, Choi EJ, Lee YJ, Zhang Y, Zhou L, Oh SP, May WS., Free PMC Article | 01/21/2010 |
| Loquacious protein is not critical for assembly of microRNA as shown in dicer-1 knockout egg extract. | Dicer-1, but not Loquacious, is critical for assembly of miRNA-induced silencing complexes.
Liu X, Park JK, Jiang F, Liu Y, McKearin D, Liu Q., Free PMC Article | 01/21/2010 |
| Both domains of Loqs-PB are required for efficient miRNA production by enhancing the affinity of Dcr-1 for pre-miRNA. | Functional anatomy of the Drosophila microRNA-generating enzyme.
Ye X, Paroo Z, Liu Q. | 01/21/2010 |
| R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development | Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila.
Jiang F, Ye X, Liu X, Fincher L, McKearin D, Liu Q., Free PMC Article | 01/21/2010 |
| findings show that normal processing of Drosophila pre-miRNAs by Dicer-1 requires the double-stranded RNA-binding domain protein Loquacious (Loqs) | Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein.
Förstemann K, Tomari Y, Du T, Vagin VV, Denli AM, Bratu DP, Klattenhoff C, Theurkauf WE, Zamore PD., Free PMC Article | 01/21/2010 |
| results indicate that Loqs and Dicer-1 form a complex that converts pre-miRNAs into mature miRNAs | Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells.
Saito K, Ishizuka A, Siomi H, Siomi MC., Free PMC Article | 01/21/2010 |
| Loqs is required intrinsically for germline stem cell maintenance. | The miRNA pathway intrinsically controls self-renewal of Drosophila germline stem cells.
Park JK, Liu X, Strauss TJ, McKearin DM, Liu Q. | 01/21/2010 |