| m(6)A RNA methylation regulates promoter- proximal pausing of RNA polymerase II. | m(6)A RNA methylation regulates promoter- proximal pausing of RNA polymerase II.
Akhtar J, Renaud Y, Albrecht S, Ghavi-Helm Y, Roignant JY, Silies M, Junion G. | 09/4/2021 |
| The C-terminal domain (CTD) of RNA polymerase II (Pol II) is composed of repeats of the consensus YSPTSPS and is an essential binding scaffold for transcription-associated factors, supporting drosophila development with consensus heptads. | The C-Terminal Domain of RNA Polymerase II Is a Multivalent Targeting Sequence that Supports Drosophila Development with Only Consensus Heptads.
Lu F, Portz B, Gilmour DS., Free PMC Article | 09/19/2020 |
| Study measured the first wave of de novo transcription in living Drosophila embryos using dual-fluorescence detection of nascent transcripts containing 5' MS2 and 3' PP7 RNA stem loops. Pol II elongation rates of 2.4-3.0 kb/min were observed, approximately twice as fast as earlier estimates | Rapid Rates of Pol II Elongation in the Drosophila Embryo.
Fukaya T, Lim B, Levine M., Free PMC Article | 08/4/2018 |
| Genetic and biochemical analyses reveal a functional interaction of MSL1 with CDK7, a subunit of the Cdk-activating kinase (CAK) complex of the general transcription factor TFIIH. Importantly, MSL1 depletion leads to decreased phosphorylation of Ser5 of RNA polymerase II. | Functional interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation.
Chlamydas S, Holz H, Samata M, Chelmicki T, Georgiev P, Pelechano V, Dündar F, Dasmeh P, Mittler G, Cadete FT, Ramírez F, Conrad T, Wei W, Raja S, Manke T, Luscombe NM, Steinmetz LM, Akhtar A. | 05/20/2017 |
| Phosphorylation of the C-terminal domain of RNA polymerase II (CTD) plays an essential role in eukaryotic transcription by recruiting transcriptional regulatory factors to the active polymerase. This study shows that Tyrosine 1 is required for CTD phosphorylation by Erk2. | Mapping the Phosphorylation Pattern of Drosophila melanogaster RNA Polymerase II Carboxyl-Terminal Domain Using Ultraviolet Photodissociation Mass Spectrometry.
Mayfield JE, Robinson MR, Cotham VC, Irani S, Matthews WL, Ram A, Gilmour DS, Cannon JR, Zhang YJ, Brodbelt JS., Free PMC Article | 02/25/2017 |
| Study provides additional evidence that Zeste protein plays no role in the activation of the white gene transcription; does not rule out that the white gene enhancer is able to recruit RNAPII and function as a weak promoter | Functional organization of the white gene enhancer in Drosophila melanogaster.
Melnikova LS, Kostyuchenko MV, Georgiev PG. | 01/25/2016 |
| This action of GAF on nucleosomes is at least partially independent of paused Pol II because intergenic GAF binding sites with little or no Pol II also show GAF-dependent nucleosome displacement. | GAGA factor maintains nucleosome-free regions and has a role in RNA polymerase II recruitment to promoters.
Fuda NJ, Guertin MJ, Sharma S, Danko CG, Martins AL, Siepel A, Lis JT., Free PMC Article | 01/2/2016 |
| Loss of dPNUTS function or specific disruption of its ability to bind PP1 results in hyperphosphorylation of the RNAPII CTD in whole animal extracts and on chromosomes | PNUTS/PP1 regulates RNAPII-mediated gene expression and is necessary for developmental growth.
Ciurciu A, Duncalf L, Jonchere V, Lansdale N, Vasieva O, Glenday P, Rudenko A, Vissi E, Cobbe N, Alphey L, Bennett D., Free PMC Article | 02/21/2015 |
| Determined are the kinetic and structural properties of Drosophila Ssu72-symplekin in complex with the C-terminal domain of RNA polymerase II peptide with consecutive phosphorylated Thr4 and Ser5. [Ssu72] | novel modifications on C-terminal domain of RNA polymerase II can fine-tune the phosphatase activity of Ssu72.
Luo Y, Yogesha SD, Cannon JR, Yan W, Ellington AD, Brodbelt JS, Zhang Y., Free PMC Article | 04/5/2014 |
| A novel single-nucleotide mutation in the 3' UTR of the RNPII215 gene leads to a reduced number of nuclear divisions that is accompanied by premature transcription of early zygotic genes and cellularization. | Number of nuclear divisions in the Drosophila blastoderm controlled by onset of zygotic transcription.
Sung HW, Spangenberg S, Vogt N, Großhans J. | 08/10/2013 |
| Cohesin depletion decreases the levels of transcriptionally engaged Pol II at the promoters of most genes that don't bind cohesin, suggesting that cohesin controls expression of one or more broadly acting general transcription factors | Genome-wide control of RNA polymerase II activity by cohesin.
Schaaf CA, Kwak H, Koenig A, Misulovin Z, Gohara DW, Watson A, Zhou Y, Lis JT, Dorsett D., Free PMC Article | 06/29/2013 |
| Hsromega-n transcripts are essential for thermotolerance and remobilization of hnRNPs, HP1 and RNA polymerase II during recovery from heat shock. | The large noncoding hsrω-n transcripts are essential for thermotolerance and remobilization of hnRNPs, HP1 and RNA polymerase II during recovery from heat shock in Drosophila.
Lakhotia SC, Mallik M, Singh AK, Ray M. | 05/19/2012 |
| analysis of the interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex | Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex.
Missra A, Gilmour DS., Free PMC Article | 07/26/2010 |
| RNA polymerase II act as dominant modifiers of the derepression phenotypes of the Sex combs reduced (Scr) and Ultrabithorax (Ubx) genes. | RNA polymerase II 140wimp mutant and mutations in the TFIIH subunit XPB differentially affect homeotic gene expression in Drosophila.
Gutiérrez L, Merino C, Vázquez M, Reynaud E, Zurita M. | 01/21/2010 |
| resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4 pol II which demonstrates the transcriptional control of alternative splicing on an endogenous gene | A slow RNA polymerase II affects alternative splicing in vivo.
de la Mata M, Alonso CR, Kadener S, Fededa JP, Blaustein M, Pelisch F, Cramer P, Bentley D, Kornblihtt AR. | 01/21/2010 |