| The results indicate that Polkappa does not suppress benzo[a]pyrene -induced mutagenesis and carcinogenesis in the colon, but counteracts inflammation-induced mutagenesis in multiple organs. | DNA polymerase kappa counteracts inflammation-induced mutagenesis in multiple organs of mice.
Hakura A, Sui H, Sonoda J, Matsuda T, Nohmi T. | 04/27/2019 |
| The results suggest that Polk has a limited ability to suppress BP-induced genotoxicity in the colon and bone marrow and also that the roles of specialized DNA polymerases in mutagenesis and carcinogenesis should be examined not only by in vitro assays but also by in vivo mouse studies. We also report the spontaneous mutagenesis in inactivated Polk KI mice at young and old ages. | Limited ability of DNA polymerase kappa to suppress benzo[a]pyrene-induced genotoxicity in vivo.
Masumura K, Toyoda-Hokaiwado N, Niimi N, Grúz P, Wada NA, Takeiri A, Jishage KI, Mishima M, Nohmi T. | 12/2/2017 |
| the extreme N-terminal part of Polkappa is required for the processivity and fidelity of Polkappa during translesion synthesis of 10S(+)-trans-anti-benzo[a]pyrene diol epoxide-N(2)-deoxyguanine adducts lesions. | Effects of the N terminus of mouse DNA polymerase κ on the bypass of a guanine-benzo[a]pyrenyl adduct.
Liu Y, Ma X, Guo C., Free PMC Article | 10/29/2016 |
| Polk plays a predominant role in suppressing point mutations by carrying out error-free translesion DNA synthesis and contributes to the prevention of DNA strand breaks. | In vivo evidence that DNA polymerase kappa is responsible for error-free bypass across DNA cross-links induced by mitomycin C.
Takeiri A, Wada NA, Motoyama S, Matsuzaki K, Tateishi H, Matsumoto K, Niimi N, Sassa A, Grúz P, Masumura K, Yamada M, Mishima M, Jishage KI, Nohmi T. | 10/24/2015 |
| The structural gap physically accommodates the bulky aromatic adduct and the N-clasp is essential for the structural integrity and flexibility of Polkappa during translesion synthesis. | Variants of mouse DNA polymerase κ reveal a mechanism of efficient and accurate translesion synthesis past a benzo[a]pyrene dG adduct.
Liu Y, Yang Y, Tang TS, Zhang H, Wang Z, Friedberg E, Yang W, Guo C., Free PMC Article | 04/5/2014 |
| Polkappa accumulates at laser-induced sites of DNA damage. | Mouse DNA polymerase kappa has a functional role in the repair of DNA strand breaks.
Zhang X, Lv L, Chen Q, Yuan F, Zhang T, Yang Y, Zhang H, Wang Y, Jia Y, Qian L, Chen B, Zhang Y, Friedberg EC, Tang TS, Guo C., Free PMC Article | 10/26/2013 |
| Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) zeta, and Pol kappa | Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ζ, and Pol κ.
Wojtaszek J, Lee CJ, D'Souza S, Minesinger B, Kim H, D'Andrea AD, Walker GC, Zhou P., Free PMC Article | 12/22/2012 |
| Data suggest that DNA polymerase kappa Polkappa functions in DNA interstrand crosslinks (ICLs) repair in embryonic fibroblast cells (MEF), especially during the G0/G1 phases. | Replication-independent repair of DNA interstrand crosslinks.
Williams HL, Gottesman ME, Gautier J., Free PMC Article | 10/27/2012 |
| solution structure of the polymerase kappa-Rev1 complex | Multifaceted recognition of vertebrate Rev1 by translesion polymerases ζ and κ.
Wojtaszek J, Liu J, D'Souza S, Wang S, Xue Y, Walker GC, Zhou P., Free PMC Article | 10/13/2012 |
| in mammalian cells, both polymerases kappa and iota are necessary for the error-free bypass of N(2)-CEdG and N(2)-CMdG. | The roles of DNA polymerases κ and ι in the error-free bypass of N2-carboxyalkyl-2'-deoxyguanosine lesions in mammalian cells.
Yuan B, You C, Andersen N, Jiang Y, Moriya M, O'Connor TR, Wang Y., Free PMC Article | 07/30/2011 |
| results are consistent with the notion that Pol kappa is required for accurate translesion DNA synthesis past naturally occurring polycyclic guanine adducts, possibly generated by cholesterol and/or its metabolites. | Polk mutant mice have a spontaneous mutator phenotype.
Stancel JN, McDaniel LD, Velasco S, Richardson J, Guo C, Friedberg EC., Free PMC Article | 02/8/2010 |
| The REV1-interaction is essential for Polkappa function in vivo. | Identification of a novel REV1-interacting motif necessary for DNA polymerase kappa function.
Ohashi E, Hanafusa T, Kamei K, Song I, Tomida J, Hashimoto H, Vaziri C, Ohmori H., Free PMC Article | 01/21/2010 |
| Polkappa binds with monoubiquitinated proliferating cell nuclear antigen (PCNA) more robustly than with non-ubiquitinated PCNA. | Requirements for the interaction of mouse Polkappa with ubiquitin and its biological significance.
Guo C, Tang TS, Bienko M, Dikic I, Friedberg EC. | 01/21/2010 |
| Polkappa plays an important role in suppressing mutations at DNA lesions generated by benzo[a]pyrene, but not UV or x-ray irradiation. | Polkappa protects mammalian cells against the lethal and mutagenic effects of benzo[a]pyrene.
Ogi T, Shinkai Y, Tanaka K, Ohmori H., Free PMC Article | 01/21/2010 |
| pol kappa-deficient mouse cells have substantially reduced (but not absent) levels of nucleotide excision repair | The Y-family DNA polymerase kappa (pol kappa) functions in mammalian nucleotide-excision repair.
Ogi T, Lehmann AR. | 01/21/2010 |
| Rev7 competes directly with Pol kappa for binding to the Rev1 C-terminus. | Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis.
Guo C, Fischhaber PL, Luk-Paszyc MJ, Masuda Y, Zhou J, Kamiya K, Kisker C, Friedberg EC., Free PMC Article | 01/21/2010 |
| The multiple mouse/human (PolK/POLK) transcripts may encode multiple Polkappa isoforms in testis | Multiple PolK (POLK) transcripts in mammalian testis.
Guo C, Gao T, Confer N, Velasco-Miguel S, Friedberg EC. | 01/21/2010 |
| compromised translesion synthesis in Polkappa(-/-) mice may result in replication fork pausing which, in turn, may affect expanded simple tandem repeat mutation rate | Elevated mutation rates in the germline of Polkappa mutant male mice.
Burr KL, Velasco-Miguel S, Duvvuri VS, McDaniel LD, Friedberg EC, Dubrova YE. | 01/21/2010 |
| Pol kappa and Pol iota double-deficient mice had the normal somatic hypermutation frequency | Normal immunoglobulin gene somatic hypermutation in Pol kappa-Pol iota double-deficient mice.
Shimizu T, Azuma T, Ishiguro M, Kanjo N, Yamada S, Ohmori H. | 01/21/2010 |
| Polk causes error-prone and inefficient replication across 8-oxoguanine in ras genes. | Error-prone and inefficient replication across 8-hydroxyguanine (8-oxoguanine) in human and mouse ras gene fragments by DNA polymerase kappa.
Jałoszyński P, Ohashi E, Ohmori H, Nishimura S. | 01/21/2010 |
| Polkappa has a function in translesion DNA synthesis past alkylated base adducts as well as UV radiation DNA damage in vertebrates | Involvement of vertebrate Polkappa in translesion DNA synthesis across DNA monoalkylation damage.
Takenaka K, Ogi T, Okada T, Sonoda E, Guo C, Friedberg EC, Takeda S. | 01/21/2010 |
| the trans-lesion synthesis enzyme polkappa is specifically required for normal recovery from the BPDE-induced S-phase checkpoint | DNA polymerase kappa is specifically required for recovery from the benzo[a]pyrene-dihydrodiol epoxide (BPDE)-induced S-phase checkpoint.
Bi X, Slater DM, Ohmori H, Vaziri C. | 01/21/2010 |