| Manipulation of a cation-pi sandwich reveals conformational flexibility in phenylalanine hydroxylase. | Manipulation of a cation-π sandwich reveals conformational flexibility in phenylalanine hydroxylase.
Arturo EC, Merkel GW, Hansen MR, Lisowski S, Almeida D, Gupta K, Jaffe EK., Free PMC Article | 06/12/2021 |
| results support a model for allostery in PheH in which phenylalanine stabilizes the dimerization of the regulatory domains and exposes the active site for substrate binding and other structural changes needed for activity | Domain Movements upon Activation of Phenylalanine Hydroxylase Characterized by Crystallography and Chromatography-Coupled Small-Angle X-ray Scattering.
Meisburger SP, Taylor AB, Khan CA, Zhang S, Fitzpatrick PF, Ando N., Free PMC Article | 05/5/2018 |
| The results identify the location of the allosteric site as the interface of the regulatory domain dimer formed in activated PheH. | Identification of the Allosteric Site for Phenylalanine in Rat Phenylalanine Hydroxylase.
Zhang S, Fitzpatrick PF., Free PMC Article | 08/20/2016 |
| use of SAXS and X-ray crystallography together to inspect PAH structure provides, to our knowledge, the first complete view of the enzyme in a tetrameric form that was not possible with prior partial crystal structures | First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer.
Arturo EC, Gupta K, Héroux A, Stith L, Cross PJ, Parker EJ, Loll PJ, Jaffe EK., Free PMC Article | 08/6/2016 |
| allosteric activation of phenylalanine hydroxylase is linked to dimerization of regulatory domains | The Amino Acid Specificity for Activation of Phenylalanine Hydroxylase Matches the Specificity for Stabilization of Regulatory Domain Dimers.
Zhang S, Hinck AP, Fitzpatrick PF., Free PMC Article | 11/7/2015 |
| Data suggest that phenylalanine [concentration-dependent] activation of phenylalanine hydroxylase does not involve the active site; mutating the active-site residue Arg270 to lysine abolishes phenylalanine-dependent enzyme activation. | Activation of phenylalanine hydroxylase by phenylalanine does not require binding in the active site.
Roberts KM, Khan CA, Hinck CS, Fitzpatrick PF., Free PMC Article | 02/21/2015 |
| the conformational change upon phosphorylation of phenylalanine hydroxylase is different from and less extensive than that upon phenylalanine activation. | Regulation of phenylalanine hydroxylase: conformational changes upon phosphorylation detected by H/D exchange and mass spectrometry.
Li J, Fitzpatrick PF., Free PMC Article | 08/31/2013 |
| A new model for allosteric regulation of phenylalanine hydroxylase: implications for disease and therapeutics. | A new model for allosteric regulation of phenylalanine hydroxylase: implications for disease and therapeutics.
Jaffe EK, Stith L, Lawrence SH, Andrake M, Dunbrack RL Jr., Free PMC Article | 04/20/2013 |
| the regulatory domain of phenylalanine hydroxylase can bind phenylalanine | Direct evidence for a phenylalanine site in the regulatory domain of phenylalanine hydroxylase.
Li J, Ilangovan U, Daubner SC, Hinck AP, Fitzpatrick PF., Free PMC Article | 02/26/2011 |
| Metal ligand mutants of Pah are characterized. | Characterization of metal ligand mutants of phenylalanine hydroxylase: Insights into the plasticity of a 2-histidine-1-carboxylate triad.
Li J, Fitzpatrick PF., Free PMC Article | 01/21/2010 |
| Promoter characterized, sequences bind HNF1 and CREB transcription factors present in rat nuclear extracts | Sequence analysis of the rat phenylalanine hydroxylase gene promoter.
Rees D, Fisher MJ, McDowall IL. | 01/21/2010 |