| High-yield recombinant bacterial expression of [13] C-, [15] N-labeled, serine-16 phosphorylated, murine amelogenin using a modified third generation genetic code expansion protocol. | High-yield recombinant bacterial expression of (13) C-, (15) N-labeled, serine-16 phosphorylated, murine amelogenin using a modified third generation genetic code expansion protocol.
Buchko GW, Zhou M, Vesely CH, Tao J, Shaw WJ, Mehl RA, Cooley RB., Free PMC Article | 02/4/2023 |
| An miRNA derived from amelogenin exon4 regulates expression of transcription factor Runx2 by directly targeting upstream activators Nfia and Prkch. | An miRNA derived from amelogenin exon4 regulates expression of transcription factor Runx2 by directly targeting upstream activators Nfia and Prkch.
Shemirani R, Lin G, Abduweli Uyghurturk D, Le M, Nakano Y., Free PMC Article | 06/11/2022 |
| Loss of biological control of enamel mineralization in amelogenin-phosphorylation-deficient mice. | Loss of biological control of enamel mineralization in amelogenin-phosphorylation-deficient mice.
Stifler CA, Yamazaki H, Gilbert PUPA, Margolis HC, Beniash E., Free PMC Article | 06/11/2022 |
| Leucine rich amelogenin peptide prevents ovariectomy-induced bone loss in mice. | Leucine rich amelogenin peptide prevents ovariectomy-induced bone loss in mice.
Haruyama N, Yamaza T, Suzuki S, Hall B, Cho A, Gibson CW, Kulkarni AB., Free PMC Article | 01/1/2022 |
| MMP20-generated amelogenin cleavage products prevent formation of fan-shaped enamel malformations. | MMP20-generated amelogenin cleavage products prevent formation of fan-shaped enamel malformations.
Bartlett JD, Smith CE, Hu Y, Ikeda A, Strauss M, Liang T, Hsu YH, Trout AH, McComb DW, Freeman RC, Simmer JP, Hu JC., Free PMC Article | 11/6/2021 |
| Stage-Specific Role of Amelx Activation in Stepwise Ameloblast Induction from Mouse Induced Pluripotent Stem Cells. | Stage-Specific Role of Amelx Activation in Stepwise Ameloblast Induction from Mouse Induced Pluripotent Stem Cells.
Miao X, Niibe K, Zhang M, Liu Z, Nattasit P, Ohori-Morita Y, Nakamura T, Jiang X, Egusa H., Free PMC Article | 07/31/2021 |
| Novel LRAP-binding partner revealing the plasminogen activation system as a regulator of cementoblast differentiation and mineral nodule formation in vitro. | Novel LRAP-binding partner revealing the plasminogen activation system as a regulator of cementoblast differentiation and mineral nodule formation in vitro.
Martins L, Amorim BR, Salmon CR, Leme AFP, Kantovitz KR, Nociti FH Jr. | 03/13/2021 |
| Protein nanoribbons template enamel mineralization. | Protein nanoribbons template enamel mineralization.
Bai Y, Yu Z, Ackerman L, Zhang Y, Bonde J, Li W, Cheng Y, Habelitz S., Free PMC Article | 10/24/2020 |
| Amelogenin phosphorylation regulates tooth enamel formation by stabilizing a transient amorphous mineral precursor. | Amelogenin phosphorylation regulates tooth enamel formation by stabilizing a transient amorphous mineral precursor.
Shin NY, Yamazaki H, Beniash E, Yang X, Margolis SS, Pugach MK, Simmer JP, Margolis HC., Free PMC Article | 10/24/2020 |
| Results show that leucine-rich amelogenin (LRAP) assemblies with different sizes all contained a consistent rigid segment with beta-sheet secondary structure and the beta-sheet segment would further assemble into amyloid-like structures. | Amyloid structure of high-order assembly of Leucine-rich amelogenin revealed by solid-state NMR.
Ma CW, Zhang J, Dong XQ, Lu JX. | 06/6/2020 |
| High-resolution, in situ atomic force microscopy (AFM) showed that altering one amino acid within the murine amelogenin sequence (natural variants T21 and P41T, and experimental variant P71T) resulted in an increase in the quantity of protein adsorbed onto hydroxyapatite (HAP) and the formation of multiple protein layers | The energetic basis for hydroxyapatite mineralization by amelogenin variants provides insights into the origin of amelogenesis imperfecta.
Tao J, Shin Y, Jayasinha R, Buchko GW, Burton SD, Dohnalkova AC, Wang Z, Shaw WJ, Tarasevich BJ., Free PMC Article | 03/28/2020 |
| The maxillary incisors of Amelx-/- mutants indicated the increase of the frequency and number of labial grooves with aging. | Amelogenin X impacts age-dependent increase of frequency and number in labial incisor grooves in C57BL/6.
Ota MS, Kondo K, Li Y, Iseki S, Yamashita A, Gibson CW, Kondo T. | 02/17/2018 |
| Results provide evidence that all three regions of amelogenin (N-terminus, C-terminus, central core) are required for enamel formation and structure. | Truncated amelogenin and LRAP transgenes improve Amelx null mouse enamel.
Xia Y, Ren A, Pugach MK., Free PMC Article | 12/23/2017 |
| an involvement of LRAP in lipid-raft-dependent signaling pathways are mediated by flotillin-1. | Leucine-Rich Amelogenin Peptide (LRAP) Uptake by Cementoblast Requires Flotillin-1 Mediated Endocytosis.
Martins L, Leme AF, Kantovitz KR, de Luciane Martins EN, Sallum EA, Casati MZ, Nociti FH Jr. | 05/20/2017 |
| transcriptional activation of Amelx was associated with enhanced osteogenic differentiation, especially in the early stage of biomineralization | Controlled Osteogenic Differentiation of Mouse Mesenchymal Stem Cells by Tetracycline-Controlled Transcriptional Activation of Amelogenin.
Wang F, Okawa H, Kamano Y, Niibe K, Kayashima H, Osathanon T, Pavasant P, Saeki M, Yatani H, Egusa H., Free PMC Article | 07/16/2016 |
| LRAP is primarily monomeric in solution, suggesting that the monomeric species may be an important functional form of some amelogenins. | The leucine-rich amelogenin protein (LRAP) is primarily monomeric and unstructured in physiological solution.
Tarasevich BJ, Philo JS, Maluf NK, Krueger S, Buchko GW, Lin G, Shaw WJ., Free PMC Article | 12/26/2015 |
| AMELX may function as a growth factor-like molecules solubilized in the aqueous microenvironment. In jaws, it might play some role in bone physiology through autocrine/paracrine pathways, particularly during development and stress-induced remodeling. | Tracking endogenous amelogenin and ameloblastin in vivo.
Jacques J, Hotton D, De la Dure-Molla M, Petit S, Asselin A, Kulkarni AB, Gibson CW, Brookes SJ, Berdal A, Isaac J., Free PMC Article | 10/24/2015 |
| temporal/spatial localization of amelogenins with exon4 peptide and functional differences in HAP binding suggests unique properties of amelogenins containing exon4 cause specific enhancement of biomineralization of early-formed HAP at maturation stage | Exon4 amelogenin transcripts in enamel biomineralization.
Stahl J, Nakano Y, Horst J, Zhu L, Le M, Zhang Y, Liu H, Li W, Den Besten PK., Free PMC Article | 08/15/2015 |
| Conversion to amelogenin expressing dental epithelial cells involved an up-regulation of the stem cell marker Sox2 and proliferation genes and decreased expression of mesenchymal markers | A pituitary homeobox 2 (Pitx2):microRNA-200a-3p:β-catenin pathway converts mesenchymal cells to amelogenin-expressing dental epithelial cells.
Sharp T, Wang J, Li X, Cao H, Gao S, Moreno M, Amendt BA., Free PMC Article | 12/20/2014 |
| Leucine rich amelogenin peptide alters ameloblast differentiation in vivo. | Leucine rich amelogenin peptide alters ameloblast differentiation in vivo.
Stahl J, Nakano Y, Kim SO, Gibson CW, Le T, DenBesten P., Free PMC Article | 07/26/2014 |
| the interaction of amelogenin with Grp78/Bip contributed to cell proliferation, rather than correlate with the osteogenic differentiation | Identification of novel amelogenin-binding proteins by proteomics analysis.
Fukuda T, Sanui T, Toyoda K, Tanaka U, Taketomi T, Uchiumi T, Nishimura F., Free PMC Article | 05/31/2014 |
| among amelogenins, M180 alone is sufficient for normal enamel mechanical properties and prism patterns, but that additional amelogenin splice products are required to restore enamel thickness. | M180 amelogenin processed by MMP20 is sufficient for decussating murine enamel.
Pugach MK, Suggs C, Li Y, Wright JT, Kulkarni AB, Bartlett JD, Gibson CW., Free PMC Article | 01/25/2014 |
| ERP expression follows defined developmental profiles; affects osteoblast differentiation, mineralization, calvarial bone development. with role in developing enamel matrix, ERPs retain conserved function related to bone biomineralization. | Expression and function of enamel-related gene products in calvarial development.
Atsawasuwan P, Lu X, Ito Y, Chen Y, Gopinathan G, Evans CA, Kulkarni AB, Gibson CW, Luan X, Diekwisch TG., Free PMC Article | 08/31/2013 |
| The study investigates the structure, orientation, and dynamics of three regions in the N-terminus of the protein: L(15) to V(19), V(19) to L(23), and K(24) to S(28). Phosphorylation and ionic strength alter the LRAP-hydroxyapatite interface. | Phosphorylation and ionic strength alter the LRAP-HAP interface in the N-terminus.
Lu JX, Xu YS, Shaw WJ., Free PMC Article | 05/25/2013 |
| Analyses of these data suggest that MMP-9 may be involved in controlling amelogenin processing and enamel formation. | Binding of amelogenin to MMP-9 and their co-expression in developing mouse teeth.
Feng J, McDaniel JS, Chuang HH, Huang O, Rakian A, Xu X, Steffensen B, Donly KJ, MacDougall M, Chen S., Free PMC Article | 04/6/2013 |