awd abnormal wing discs [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: awdprovided by FlyBase
Official Full Name: abnormal wing discsprovided by FlyBase
Primary source: FLYBASE:FBgn0000150
Locus tag: Dmel_CG2210
See related: AllianceGenome:FB:FBgn0000150
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 1084/08; anon-EST:Liang-2.27; anon-EST:Liang-2.28; anon-WO0172774.80; anon-WO0172774.82; Awd; AWD; BcDNA:GM19775; BcDNA:RH27794; CG2210; clone 2.27; clone 2.28; Dmel\CG2210; e(shi)A; eshiA; K-pn; Kpn; l(3)j2A4; l(3)L8700; NDKB; NDPK; Nm23; Nm23/awd
Summary: Enables several functions, including magnesium ion binding activity; nucleoside diphosphate kinase activity; and purine ribonucleoside triphosphate binding activity. Involved in several processes, including establishment or maintenance of polarity of follicular epithelium; nucleoside triphosphate biosynthetic process; and open tracheal system development. Located in nuclear microtubule. Is expressed in several structures, including Malpighian tubule primordium; embryonic/larval midgut primordium; follicle cell; germline cell; and larval somatic muscle cell. Human ortholog(s) of this gene implicated in neuroblastoma; ovary epithelial cancer; and teratoma. Orthologous to several human genes including NME2 (NME/NM23 nucleoside diphosphate kinase 2) and NME1-NME2 (NME1-NME2 readthrough). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 100D2-100D2; 3-102 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (31745172..31746077, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (27570894..27571799, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Impact of Drosophila Awd/NME1/2 Levels on Notch and Wg Signaling Pathways.
Title: The Impact of Drosophila Awd/NME1/2 Levels on Notch and Wg Signaling Pathways.
BMP type I receptor Thickveins (Tkv) internalization is facilitated by the Drosophila homolog of Nm23 (abnormal wing disks, AWD), which is upregulated in active intestinal stem cells (ISCs) by JNK signaling. The AWD-facilitated endocytosis of Tkv is critical for the return of ISCs to quiescence, to prevent epithelial dysplasia, and for host survival during acute intestinal infection.
Title: AWD regulates timed activation of BMP signaling in intestinal stem cells to maintain tissue homeostasis.
Down-regulation of Shi activity enhances extracellular Awd Drosophila cell lines.
Title: Dynamin controls extracellular level of Awd/Nme1 metastasis suppressor protein.
Interaction between Nm23 and the tumor suppressor VHL
Title: Interaction between Nm23 and the tumor suppressor VHL.
Awd function is required before gamma-secretase mediated cleavage since over-expression of the constitutively active form of the Notch receptor in awd mutant follicle cells allows rescue of the signaling.
Title: Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23.
Data show that awd modulates the level and localization of adherens junction components via endocytosis, and is involved in regulating morphogenesis.
Title: The Drosophila metastasis suppressor gene Nm23 homolog, awd, regulates epithelial integrity during oogenesis.
Awd is a negative regulator of ovary border-cell migration. Down-regulation allows optimal spatial signaling via Pvr & JAK/STAT. Overexpression stalls migration & reverts the phenotype of overexpressing constitutive Pvr or dominant-negative dynamin.
Title: Awd, the homolog of metastasis suppressor gene Nm23, regulates Drosophila epithelial cell invasion.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleoside diphosphate kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleoside diphosphate kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleoside diphosphate kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleoside diphosphate kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in CTP biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in CTP biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in GTP biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in GTP biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in UTP biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in UTP biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in adherens junction organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in deoxyribonucleoside triphosphate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in epithelial cell migration, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of polarity of follicular epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule-based process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleotide metabolic process	TAS Traceable Author Statement more info	PubMed
involved_in open tracheal system development	IEP Inferred from Expression Pattern more info	PubMed
involved_in open tracheal system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of tube architecture, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribonucleoside triphosphate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISS Inferred from Sequence or Structural Similarity more info
located_in nuclear microtubule	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: abnormal wing discs

Names: CG2210-PC; CG2210-PD; CG2210-PE; Killer-of-prune; NDP kinase; abnormal wing disc; abnormal wings disc; awd-PC; awd-PD; awd-PE; killer of prune; nucleoside diphosphate kinase

NP_001287624.1

EC 2.7.4.6

NP_001287625.1

EC 2.7.4.6

NP_476761.3

EC 2.7.4.6

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

31745172..31746077 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001300696.1 → NP_001287625.1 abnormal wing discs, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001287625.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4LHX6

Conserved Domains (2) summary

PTZ00093
Location:19 → 167

PTZ00093; nucleoside diphosphate kinase, cytosolic; Provisional

pfam00334
Location:21 → 155

NDK; Nucleoside diphosphate kinase
NM_057413.4 → NP_476761.3 abnormal wing discs, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476761.3

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4LHX6

Conserved Domains (2) summary

PTZ00093
Location:19 → 167

PTZ00093; nucleoside diphosphate kinase, cytosolic; Provisional

pfam00334
Location:21 → 155

NDK; Nucleoside diphosphate kinase
NM_001300695.1 → NP_001287624.1 abnormal wing discs, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001287624.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P08879, Q9V9U5

UniProtKB/TrEMBL

A0A0B4LJ12

Conserved Domains (1) summary

PTZ00093
Location:4 → 152

PTZ00093; nucleoside diphosphate kinase, cytosolic; Provisional