STE14 protein-S-isoprenylcysteine carboxyl O-methyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: STE14
Official Full Name: protein-S-isoprenylcysteine carboxyl O-methyltransferase
Primary source: SGD:S000002818
Locus tag: YDR410C
See related: AllianceGenome:SGD:S000002818; FungiDB:YDR410C; VEuPathDB:YDR410C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity. Involved in C-terminal protein methylation and peptide pheromone maturation. Located in endoplasmic reticulum membrane and nuclear inner membrane. Orthologous to human ICMT (isoprenylcysteine carboxyl methyltransferase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See STE14 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1292372..1293091, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

proof that Ste14p is the sole component necessary for the carboxylmethylation of isoprenylated substrates, also methyates Ras2p, does not have a preference for the farnesyl or geranylgeranyl moieties in the model substrates
Title: Purification, functional reconstitution, and characterization of the Saccharomyces cerevisiae isoprenylcysteine carboxylmethyltransferase Ste14p.
Effect on the functional reconstitution of Ste14p, a membrane-associated isoprenylcysteine carboxyl methyltransferase from Saccharomyces cerevisiae., has benn tested with three bipolar archaeal-type diglycerophosphocholine tetraether lipids.
Title: Functional reconstitution of the integral membrane enzyme, isoprenylcysteine carboxyl methyltransferase, in synthetic bolalipid membrane vesicles.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in C-terminal protein methylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in peptide pheromone maturation	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptide pheromone maturation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to pheromone	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear inner membrane	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: protein-S-isoprenylcysteine carboxyl O-methyltransferase

NP_010698.1

EC 2.1.1.100

Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1292372..1293091 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180718.1 → NP_010698.1 TPA: protein-S-isoprenylcysteine carboxyl O-methyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010698.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VT42, P32584

UniProtKB/TrEMBL

A6ZZ09, B3LFP4, B5VGU2, C7GY33, C8Z5X3, N1PAJ6

Conserved Domains (1) summary

COG2020
Location:50 → 239

STE14; Protein-S-isoprenylcysteine O-methyltransferase Ste14 [Posttranslational modification, protein turnover, chaperones]