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    bru1 bruno 1 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 34648, updated on 9-Dec-2024

    Summary

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    Official Symbol
    bru1provided by FlyBase
    Official Full Name
    bruno 1provided by FlyBase
    Primary source
    FLYBASE:FBgn0000114
    Locus tag
    Dmel_CG31762
    See related
    AllianceGenome:FB:FBgn0000114
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    aret; Aret; ARET; aret/bruno1; arrest/Bruno; BcDNA:GM15173; bru; Bru; bru-1; Bru1; bruno; Bruno; CG31762; CG6319; Dm Bru; Dmel\CG31762; ms(2)01284; NEST:bs01g04; NEST:bs34h11
    Summary
    Enables mRNA 3'-UTR binding activity. Involved in several processes, including female germ-line stem cell population maintenance; positive regulation of exit from mitosis; and regulation of gene expression. Located in P granule; nuclear body; and nuclear membrane. Is expressed in several structures, including adult head; embryonic anal pad; flight muscle cell; germline cell; and gonad. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 97. Orthologous to human CELF1 (CUGBP Elav-like family member 1) and CELF2 (CUGBP Elav-like family member 2). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See bru1 in Genome Data Viewer
    Location:
    33D2-33E1; 2-46 cM
    Exon count:
    20
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2L NT_033779.5 (12175004..12313438)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2L NT_033779.4 (12175004..12313438)

    Chromosome 2L - NT_033779.5Genomic Context describing neighboring genes Neighboring gene long non-coding RNA:CR43356 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene atilla Neighboring gene crooked Neighboring gene uncharacterized protein Neighboring gene long non-coding RNA:CR45282 Neighboring gene long non-coding RNA:CR45283

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Characterization of an invertebrate nicotinic acetylcholine receptor gene: the ard gene of Drosophila melanogaster. Sawruk E, et al. FEBS Lett, 1988 Aug 1. PMID 3136037
    2. A compendium of RNA-binding motifs for decoding gene regulation. Ray D, et al. Nature, 2013 Jul 11. PMID 23846655, Free PMC Article
    3. Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression. Reveal B, et al. RNA Biol, 2011 Nov-Dec. PMID 21955496, Free PMC Article
    4. Bruno protein contains an expanded RNA recognition motif. Lyon AM, et al. Biochemistry, 2009 Dec 29. PMID 19919093, Free PMC Article
    5. From factors to mechanisms: translation and translational control in eukaryotes. Preiss T, et al. Curr Opin Genet Dev, 1999 Oct. PMID 10508691

    See all (213) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Bruno 1/CELF regulates splicing and cytoskeleton dynamics to ensure correct sarcomere assembly in Drosophila flight muscles.
      Title: Bruno 1/CELF regulates splicing and cytoskeleton dynamics to ensure correct sarcomere assembly in Drosophila flight muscles.
    2. Results identify a novel regulatory circuit in drosophila, with Bru restricting the activity of osk, and osk in turn restricting the activity of Bru.
      Title: oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction.
    3. Here we describe an in vivo assay system to monitor the spatial pattern of Bru-dependent repression, separate from the full complexity of osk regulation.
      Title: Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression.
    4. Aret potently promotes flight muscle splicing patterns when ectopically expressed in Drosophila jump muscles.
      Title: Arrest is a regulator of fiber-specific alternative splicing in the indirect flight muscles of Drosophila.
    5. A role for Bruno in Enok-controlled GSC maintenance
      Title: The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche.
    6. Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression.(
      Title: Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression.
    7. Bruno RNA recognition motif contains four antiparallel beta-strands and two alpha-helices.
      Title: Bruno protein contains an expanded RNA recognition motif.
    8. Repression by Bruno during oogenesis is required to restrict Gcl expression in the early embryo.
      Title: Bruno negatively regulates germ cell-less expression in a BRE-independent manner.
    9. Bruno shuttles between the nucleus and cytoplasm, and may first bind oskar mRNA in the nucleus.
      Title: Two distinct domains of Bruno bind specifically to the oskar mRNA.
    10. Ectopic expression of bruno prolongs oskar repression and blocks posterior body patterning.
      Title: A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    mobile_element

    • Loc: 12197314-12197348 mobile_element_type = transposon:INE-1{}2179
    • Loc: 12262559-12262626 mobile_element_type = transposon:H{}2183
    • Loc: 12285973-12286003 mobile_element_type = transposon:INE-1{}2181

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables mRNA 3'-UTR binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables mRNA 3'-UTR binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables mRNA 3'-UTR binding TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables mRNA binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables mRNA binding NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    enables mRNA binding TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in female germ-line stem cell population maintenance IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in germ cell development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA splice site recognition IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of oskar mRNA translation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of oskar mRNA translation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in negative regulation of oskar mRNA translation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in negative regulation of oskar mRNA translation TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in negative regulation of translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in oogenesis HMP PubMed 
    involved_in oogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of exit from mitosis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of stem cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of alternative mRNA splicing, via spliceosome HMP PubMed 
    involved_in regulation of alternative mRNA splicing, via spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of alternative mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of myofibril size IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in sarcomere organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in P granule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nuclear body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nuclear membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IC
    Inferred by Curator
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    part_of ribonucleoprotein complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    bruno 1
    Names
    CG31762-PA
    CG31762-PB
    CG31762-PD
    CG31762-PE
    CG31762-PF
    CG31762-PG
    CG31762-PH
    CG31762-PI
    CG31762-PJ
    CG31762-PK
    CG31762-PL
    arrest
    bru1-PA
    bru1-PB
    bru1-PD
    bru1-PE
    bru1-PF
    bru1-PG
    bru1-PH
    bru1-PI
    bru1-PJ
    bru1-PK
    bru1-PL
    bruno

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033779.5 Reference assembly

      Range
      12175004..12313438
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001273478.1 → NP_001260407.1  bruno 1, isoform K [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001260407.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      M9PDB0, O02374
      Conserved Domains (4) summary
      cd12631
      Location:119 → 200
      RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
      cd12640
      Location:485 → 563
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:122 → 571
      ELAV_HUD_SF; ELAV/HuD family splicing factor
      cl17169
      Location:206 → 287
      RRM_SF; RNA recognition motif (RRM) superfamily

    2. NM_001273477.1 → NP_001260406.1  bruno 1, isoform J [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001260406.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      M9PDB0, O02374
      Conserved Domains (4) summary
      cd12631
      Location:119 → 200
      RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
      cd12640
      Location:485 → 563
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:122 → 571
      ELAV_HUD_SF; ELAV/HuD family splicing factor
      cl17169
      Location:206 → 287
      RRM_SF; RNA recognition motif (RRM) superfamily

    3. NM_165002.2 → NP_723737.1  bruno 1, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_723737.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      C8VV87, Q8IP90
      Related
      FBpp0079926
      Conserved Domains (4) summary
      cd12631
      Location:356 → 437
      RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
      cd12636
      Location:443 → 524
      RRM2_Bruno_like; RNA recognition motif 2 in Drosophila melanogaster Bruno protein and similar proteins
      cd12640
      Location:722 → 800
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:359 → 808
      ELAV_HUD_SF; ELAV/HuD family splicing factor

    4. NM_001414076.1 → NP_001400994.1  bruno 1, isoform L [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A9F2H0X2

    5. NM_001273475.1 → NP_001260404.1  bruno 1, isoform H [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001260404.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      M9PDB0, O02374
      Conserved Domains (4) summary
      cd12631
      Location:119 → 200
      RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
      cd12640
      Location:485 → 563
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:122 → 571
      ELAV_HUD_SF; ELAV/HuD family splicing factor
      cl17169
      Location:206 → 287
      RRM_SF; RNA recognition motif (RRM) superfamily

    6. NM_001273476.1 → NP_001260405.1  bruno 1, isoform I [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001260405.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      M9PDB0, O02374
      Conserved Domains (4) summary
      cd12631
      Location:119 → 200
      RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
      cd12640
      Location:485 → 563
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:122 → 571
      ELAV_HUD_SF; ELAV/HuD family splicing factor
      cl17169
      Location:206 → 287
      RRM_SF; RNA recognition motif (RRM) superfamily

    7. NM_001273474.1 → NP_001260403.1  bruno 1, isoform G [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001260403.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      C8VV87, Q8IP90
      Conserved Domains (4) summary
      cd12631
      Location:356 → 437
      RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
      cd12636
      Location:443 → 524
      RRM2_Bruno_like; RNA recognition motif 2 in Drosophila melanogaster Bruno protein and similar proteins
      cd12640
      Location:722 → 800
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:359 → 808
      ELAV_HUD_SF; ELAV/HuD family splicing factor

    8. NM_165003.3 → NP_723738.2  bruno 1, isoform F [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_723738.2

      Status: REVIEWED

      UniProtKB/TrEMBL
      O02374, Q8IP89
      Conserved Domains (4) summary
      cd12631
      Location:150 → 231
      RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
      cd12640
      Location:532 → 610
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:153 → 618
      ELAV_HUD_SF; ELAV/HuD family splicing factor
      cl17169
      Location:237 → 318
      RRM_SF; RNA recognition motif (RRM) superfamily

    9. NM_165004.3 → NP_723739.1  bruno 1, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_723739.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      O02374, Q960Z4
      Related
      FBpp0079927
      Conserved Domains (4) summary
      cd12631
      Location:150 → 231
      RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
      cd12640
      Location:516 → 594
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:153 → 602
      ELAV_HUD_SF; ELAV/HuD family splicing factor
      cl17169
      Location:237 → 318
      RRM_SF; RNA recognition motif (RRM) superfamily

    10. NM_001169492.2 → NP_001162963.1  bruno 1, isoform E [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001162963.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      O02374, Q960Z4
      Conserved Domains (4) summary
      cd12631
      Location:150 → 231
      RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
      cd12640
      Location:516 → 594
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:153 → 602
      ELAV_HUD_SF; ELAV/HuD family splicing factor
      cl17169
      Location:237 → 318
      RRM_SF; RNA recognition motif (RRM) superfamily

    11. NM_165005.3 → NP_723740.1  bruno 1, isoform D [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_723740.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      G7H844, Q8SYC0
      Related
      FBpp0079929
      Conserved Domains (3) summary
      cd12640
      Location:273 → 351
      RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
      TIGR01661
      Location:2 → 359
      ELAV_HUD_SF; ELAV/HuD family splicing factor
      cl17169
      Location:1 → 75
      RRM_SF; RNA recognition motif (RRM) superfamily
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    M9PDB0 M9PDB0
    O02374 O02374
    O18409 O18409
    Q8IP89 Q8IP89
    Q8IP90 Q8IP90
    Q8MSG0 Q8MSG0
    Q8SYC0 Q8SYC0
    Q960Z4 Q960Z4

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