RUB1 uncharacterized protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: RUB1
Official Full Name: uncharacterized protein
Primary source: TAIR:AT1G31340
Locus tag: AT1G31340
See related: Araport:AT1G31340
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA RELATED TO UBIQUITIN 1; ATRUB1; NEDD8; related to ubiquitin 1; T19E23.13; T19E23_13
Summary: Encodes a ubiquitin-related protein that is conjugated to target proteins by neddylation. It has been shown to be conjugated to the cullin AtCUL1. The RUB-conjugation pathway has been implicated in in auxin response.
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Genomic context

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See RUB1 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 3

Sequence:: Chromosome: 1; NC_003070.9 (11217695..11219618, complement)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

AXR1 and other neddylation enzymes are autoneddylated in an E2- and E3-independent manner in vitro and that AXR1 autoneddylation impairs NEDD8 E2 approximately thioester formation in the den1 mutant background in vivo.
Title: DENEDDYLASE1 Protein Counters Automodification of Neddylating Enzymes to Maintain NEDD8 Protein Homeostasis in Arabidopsis.
mutants of a DEN1 homolog from Arabidopsis thaliana have no detectable defects in NEDD8 processing but do accumulate a broad range of NEDD8 conjugates.
Title: DENEDDYLASE1 deconjugates NEDD8 from non-cullin protein substrates in Arabidopsis thaliana.
DDB1a is an novel RUB-modified protein.
Title: Recovery of DDB1a (damaged DNA binding protein1a) in a screen to identify novel RUB-modified proteins in Arabidopsis thaliana.
AXR1 and AXL1 have similar abilities to function with E1 C-TERMINAL-RELATED1 (ECR1) in catalyzing RUB1 activation and RUB1-ECR1 thioester formation.
Title: AXR1-ECR1 and AXL1-ECR1 heterodimeric RUB-activating enzymes diverge in function in Arabidopsis thaliana.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_102873.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within embryo development ending in seed dormancy	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within ethylene biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein neddylation	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within response to auxin	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: uncharacterized protein

NP_564379.2

related to ubiquitin 1 (RUB1); INVOLVED IN: protein modification process, response to auxin stimulus, protein neddylation, ethylene biosynthetic process, embryo development; LOCATED IN: plasma membrane; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ubiquitin subgroup (InterPro:IPR019956), Ubiquitin conserved site (InterPro:IPR019954), Ubiquitin (InterPro:IPR000626), Ubiquitin supergroup (InterPro:IPR019955); BEST Arabidopsis thaliana protein match is: ubiquitin 7 (TAIR:AT2G35635.1); Has 16064 Blast hits to 7349 proteins in 721 species: Archae - 0; Bacteria - 36; Metazoa - 7330; Fungi - 1879; Plants - 3570; Viruses - 331; Other Eukaryotes - 2918 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

11217695..11219618 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_102873.5 → NP_564379.2 uncharacterized protein AT1G31340 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_564379.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WQU3, Q9SHE7

UniProtKB/TrEMBL

A0A178W1F2, A0A5S9WJT2, A0A7G2DX55

Conserved Domains (2) summary

cd01803
Location:1 → 76

Ubl_ubiquitin; ubiquitin-like (Ubl) domain found in ubiquitin

cd01806
Location:79 → 152

Ubl_NEDD8; ubiquitin-like (Ubl) domain found in neural precursor cell expressed developmentally down-regulated protein 8 (NEDD8) and similar proteins