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    leuA 2-isopropylmalate synthase [ Mycobacterium tuberculosis H37Rv ]

    Gene ID: 885092, updated on 6-Oct-2023

    Summary

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    Gene symbol
    leuA
    Gene description
    2-isopropylmalate synthase
    Locus tag
    Rv3710
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Mycobacterium tuberculosis H37Rv (strain: H37Rv, type-material: type strain of Mycobacterium tuberculosis)
    Lineage
    Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex
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    Genomic context

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    Sequence:
    NC_000962.3 (4153740..4155674)

    NC_000962.3Genomic Context describing neighboring genes Neighboring gene aspartate-semialdehyde dehydrogenase Neighboring gene aspartokinase Neighboring gene DNA polymerase III subunit epsilon Neighboring gene ligase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000962.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mechanistic and bioinformatic investigation of a conserved active site helix in α-isopropylmalate synthase from Mycobacterium tuberculosis, a member of the DRE-TIM metallolyase superfamily. Casey AK, et al. Biochemistry, 2014 May 13. PMID 24720347, Free PMC Article
    2. Kinetic evidence for interdomain communication in the allosteric regulation of alpha-isopropylmalate synthase from Mycobacterium tuberculosis. de Carvalho LP, et al. Biochemistry, 2009 Mar 10. PMID 19166329, Free PMC Article
    3. Kinetic and chemical mechanism of alpha-isopropylmalate synthase from Mycobacterium tuberculosis. de Carvalho LP, et al. Biochemistry, 2006 Jul 25. PMID 16846242, Free PMC Article
    4. TubercuList--10 years after. Lew JM, et al. Tuberculosis (Edinb), 2011 Jan. PMID 20980199
    5. Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv. Camus JC, et al. Microbiology (Reading), 2002 Oct. PMID 12368430

    See all (6) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. comparison of results with previous biochemical data indicates an active site arginine residue (R80 in IPMS) is required for activity across the superfamily, suggesting that it plays a key role in catalysis, most likely through enolate stabilization
      Title: Mechanistic and bioinformatic investigation of a conserved active site helix in α-isopropylmalate synthase from Mycobacterium tuberculosis, a member of the DRE-TIM metallolyase superfamily.
    2. Data show that the Y410F mutant form of MtIPMS is insensitive to l-leucine inhibition, suggesting that this residue has completely uncoupled the inhibitory signal to the active site.
      Title: Kinetic evidence for interdomain communication in the allosteric regulation of alpha-isopropylmalate synthase from Mycobacterium tuberculosis.
    3. The alpha-keto acid specificity of the enzyme is narrow, and the acyl-CoA specificity is absolute for AcCoA. In the absence of alpha-KIV, MtIPMS does not enolize the alpha protons of AcCoA but slowly hydrolyzes acyl-CoA analogues.
      Title: Kinetic and chemical mechanism of alpha-isopropylmalate synthase from Mycobacterium tuberculosis.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    General protein information

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    Preferred Names
    2-isopropylmalate synthase
    NP_218227.3
    • alpha-isopropylmalate synthase) (alpha-IPM synthetase) (IPMS)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000962.3 Reference assembly

      Range
      4153740..4155674
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_218227.3 2-isopropylmalate synthase [Mycobacterium tuberculosis H37Rv]

      See identical proteins and their annotated locations for NP_218227.3

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A9P2H494
      Conserved Domains (1) summary
      PRK03739
      Location:39 → 644
      PRK03739; 2-isopropylmalate synthase; Validated
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    3HPS_A.1
    3HPS_B.1
    3HPZ_A.1
    3HPZ_B.1
    3HQ1_A.1
    3HQ1_B.1
    P9WQB3.1

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