Secisbp2 SECIS binding protein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Secisbp2provided by MGI
Official Full Name: SECIS binding protein 2provided by MGI
Primary source: MGI:MGI:1922670
See related: Ensembl:ENSMUSG00000035139 AllianceGenome:MGI:1922670
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SBP2; 2210413N07Rik; 2810012K13Rik
Summary: The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in a similar gene in human have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
Expression: Ubiquitous expression in thymus adult (RPKM 8.0), spleen adult (RPKM 6.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 A5; 13 26.12 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (51805721..51838080)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (51651682..51684044)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.
Title: Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.
Role of the Thyroid Gland in Expression of the Thyroid Phenotype of Sbp2-Deficient Mice.
Title: Role of the Thyroid Gland in Expression of the Thyroid Phenotype of Sbp2-Deficient Mice.
R543Q substitution resulted in residual activity and caused reduced selenoprotein translation. R543Q variant was thermally unstable in vitro and completely degraded in the mouse liver in vivo, while being partially functional in the brain.
Title: Ribosome profiling of selenoproteins in vivo reveals consequences of pathogenic Secisbp2 missense mutations.
This study identified SBP2 in adipose tissue macrophages as a potential target in rescuing insulin resistance in obesity.
Title: SBP2 deficiency in adipose tissue macrophages drives insulin resistance in obesity.
Selenocysteine insertion sequence binding protein 2 (SBP2)conditional knockout (iCKO) mice replicate the thyroid phenotype of SBP2 deficiency for investigating selenoprotein biology relevant to human disease.
Title: Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.
The results demonstrate that Secisbp2 is not strictly required for Sec incorporation and has a distinct role in stabilizing mRNAs that can be separated from its effects on UGA-redefinition
Title: The RNA-binding protein Secisbp2 differentially modulates UGA codon reassignment and RNA decay.
Secisbp2 is essential for embryonic development and enhances selenoprotein expression.
Title: Secisbp2 is essential for embryonic development and enhances selenoprotein expression.
SBP2/SECIS complex activates eEFSec by directing functional interactions between Domain IV and the ribosome to promote Sec-tRNA(Sec) binding and accommodation into the ribosomal A-site
Title: The selenocysteine-specific elongation factor contains a novel and multi-functional domain.
Selenocysteine insertion sequence (SECIS)-binding protein 2 alters conformational dynamics of residues involved in tRNA accommodation in 80 S ribosomes.
Title: Selenocysteine insertion sequence (SECIS)-binding protein 2 alters conformational dynamics of residues involved in tRNA accommodation in 80 S ribosomes.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (5) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA binding	ISO Inferred from Sequence Orthology more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ribonucleoprotein complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables ribonucleoprotein complex binding	IDA Inferred from Direct Assay more info	PubMed
enables selenocysteine insertion sequence binding	IBA Inferred from Biological aspect of Ancestor more info
enables selenocysteine insertion sequence binding	IDA Inferred from Direct Assay more info	PubMed
enables selenocysteine insertion sequence binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within RNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within forebrain neuron development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mRNA stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of selenocysteine incorporation	ISO Inferred from Sequence Orthology more info
involved_in selenocysteine incorporation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within selenocysteine incorporation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in selenocysteine incorporation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within striatum development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within translation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ribonucleoprotein complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: selenocysteine insertion sequence-binding protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001308448.1 → NP_001295377.1 selenocysteine insertion sequence-binding protein 2 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (2) that has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AC126547, AK012732, AK168634, BP760396, CA329104

Conserved Domains (1) summary

pfam01248
Location:598 → 698

Ribosomal_L7Ae; Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
NM_029279.2 → NP_083555.1 selenocysteine insertion sequence-binding protein 2 isoform 1

See identical proteins and their annotated locations for NP_083555.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC126547, AK168634, BP760396, CA329104

Consensus CDS

CCDS36665.1

UniProtKB/Swiss-Prot

Q3TGQ4, Q3U1C4

Related

ENSMUSP00000045740.9, ENSMUST00000040117.15

Conserved Domains (1) summary

pfam01248
Location:662 → 762

Ribosomal_L7Ae; Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

51805721..51838080

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006516966.3 → XP_006517029.1 selenocysteine insertion sequence-binding protein 2 isoform X1

Conserved Domains (1) summary

pfam01248
Location:365 → 465

Ribosomal_L7Ae; Ribosomal protein L7Ae/L30e/S12e/Gadd45 family