Insc INSC spindle orientation adaptor protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Inscprovided by MGI
Official Full Name: INSC spindle orientation adaptor proteinprovided by MGI
Primary source: MGI:MGI:1917942
See related: Ensembl:ENSMUSG00000048782 AllianceGenome:MGI:1917942
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: C730020D23; 3830422K02Rik
Summary: Predicted to enable cytoskeletal anchor activity and protein domain specific binding activity. Predicted to be involved in several processes, including apical protein localization; establishment of mitotic spindle orientation; and regulation of asymmetric cell division. Predicted to be located in plasma membrane. Predicted to be part of protein-containing complex. Predicted to be active in apical cortex. Predicted to colocalize with cell cortex. Is expressed in bone; lung epithelium; metanephros; and nervous system. Orthologous to human INSC (INSC spindle orientation adaptor protein). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in liver adult (RPKM 5.3), liver E18 (RPKM 3.5) and 21 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F1; 7 60.23 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (114342931..114449615)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (114743688..114850380)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Excess mINSC drives symmetric cell division, more stem cells and enhanced mammary outgrowth.
Title: Mammary Stem Cell Self-Renewal Is Regulated by Slit2/Robo1 Signaling through SNAI1 and mINSC.
Data (including data from studies using embryonic stem cell line) suggest that expression of Insc (inscuteable cell cycle protein) is up-regulated by c-Rel in cell differentiation; minimum transcriptional regulatory element (354 bases) was identified.
Title: c-Rel Regulates Inscuteable Gene Expression during Mouse Embryonic Stem Cell Differentiation.
support for the hitherto untested model that Par3-mInsc and Galphai3 act cooperatively to polarize LGN and promote perpendicular divisions
Title: Par3-mInsc and Gαi3 cooperate to promote oriented epidermal cell divisions through LGN.
Insc controls directional migration via noncanonical G protein signaling, in which Gbetagamma-free Galphai-GDP, a product from Galphai-GTP released after receptor activation, plays a central role.
Title: The cell polarity protein mInsc regulates neutrophil chemotaxis via a noncanonical G protein signaling pathway.
Mouse inscuteable induces apical-basal spindle orientation, which affects the rate of indirect neurogenesis, a process where progenitors give rise to basal progenitors, which in turn divide symmetrically into two differentiating neurons.
Title: Mouse inscuteable induces apical-basal spindle orientation to facilitate intermediate progenitor generation in the developing neocortex.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cytoskeletal anchor activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein domain specific binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein-macromolecule adaptor activity	IEA Inferred from Electronic Annotation more info
enables protein-macromolecule adaptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in apical protein localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in asymmetric cell division	IEA Inferred from Electronic Annotation more info
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in establishment of mitotic spindle orientation	IBA Inferred from Biological aspect of Ancestor more info
involved_in nervous system development	IEA Inferred from Electronic Annotation more info
involved_in regulation of asymmetric cell division	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of protein stability	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein stability	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in apical cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IEA Inferred from Electronic Annotation more info
colocalizes_with cell cortex	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein inscuteable homolog

Names: inscuteable homolog; minsc

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001405688.1 → NP_001392617.1 protein inscuteable homolog isoform 1

Status: VALIDATED

Source sequence(s)

AC102792, AC110186

UniProtKB/Swiss-Prot

B2RTA5, Q3HNM7, Q8C7I7

Related

ENSMUSP00000125061.3, ENSMUST00000161800.3
NM_001405689.1 → NP_001392618.1 protein inscuteable homolog isoform 2

Status: VALIDATED

Source sequence(s)

AC102792, AC110186

UniProtKB/TrEMBL

D3Z267, G3UW77
NM_001405690.1 → NP_001392619.1 protein inscuteable homolog isoform 3

Status: VALIDATED

Source sequence(s)

AC102792, AC110186
NM_001405691.1 → NP_001392620.1 protein inscuteable homolog isoform 3

Status: VALIDATED

Source sequence(s)

AC102792, AC110186
NM_173767.5 → NP_776128.2 protein inscuteable homolog isoform 2

See identical proteins and their annotated locations for NP_776128.2

Status: VALIDATED

Source sequence(s)

AC102792, AC110186

Consensus CDS

CCDS40095.2

UniProtKB/TrEMBL

D3Z267, G3UW77

Related

ENSMUSP00000119459.3, ENSMUST00000136645.9

Conserved Domains (1) summary

pfam16748
Location:23 → 66

INSC_LBD; Inscuteable LGN-binding domain

RNA

NR_175961.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC102792, AC110186
NR_175962.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC102792, AC110186

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

114342931..114449615

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006507662.5 → XP_006507725.1 protein inscuteable homolog isoform X2

Conserved Domains (1) summary

pfam16748
Location:70 → 112

INSC_LBD; Inscuteable LGN-binding domain
XM_036153000.1 → XP_036008893.1 protein inscuteable homolog isoform X3

UniProtKB/TrEMBL

D3Z267, G3UW77

Conserved Domains (1) summary

pfam16748
Location:23 → 66

INSC_LBD; Inscuteable LGN-binding domain
XM_006507663.5 → XP_006507726.1 protein inscuteable homolog isoform X3

See identical proteins and their annotated locations for XP_006507726.1

UniProtKB/TrEMBL

D3Z267, G3UW77

Related

ENSMUSP00000112682.2, ENSMUST00000117543.3

Conserved Domains (1) summary

pfam16748
Location:23 → 66

INSC_LBD; Inscuteable LGN-binding domain